Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 Jan;75(1):500–504. doi: 10.1073/pnas.75.1.500

Membrane particle aggregates in innervated and noninnervated cultures of Xenopus embryonic muscle cells.

H B Peng, Y Nakajima
PMCID: PMC411278  PMID: 272667

Abstract

Clusters of membrane particle aggregates were found in the cultures of Xenopus embryonic muscle cells. In innervated cultures, the aggregates were usually found in the vicinity of the nerve. In terms of particle density and morphology, they resembled the postsynaptic particle aggregates of adult skeletal muscle fibers, suggesting that they may be related to acetylcholine receptors. Similar particle aggregates were also found in noninnervated cultures. They may correspond to extrajunctional clusters of acetylcholine receptors or "hot spots."

Full text

PDF
500

Images in this article

501Image
on p.501
502Image
on p.502
503Image
on p.503

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson M. J., Cohen M. W. Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells. J Physiol. 1977 Jul;268(3):757–773. doi: 10.1113/jphysiol.1977.sp011880. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson M. J., Cohen M. W., Zorychta E. Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells. J Physiol. 1977 Jul;268(3):731–756. doi: 10.1113/jphysiol.1977.sp011879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bekoff A., Betz W. J. Acetylcholine hot spots: development on myotubes cultured from aneural limb buds. Science. 1976 Sep 3;193(4256):915–917. doi: 10.1126/science.948754. [DOI] [PubMed] [Google Scholar]
  4. Blackshaw S., Warner A. Onset of acetylcholine sensitivity and endplate activity in developing myotome muscles of Xenopus. Nature. 1976 Jul 15;262(5565):217–218. doi: 10.1038/262217a0. [DOI] [PubMed] [Google Scholar]
  5. Changeux J. P., Benedetti L., Bourgeois J. P., Brisson A., Cartaud J., Devaux P., Grünhagen H., Moreau M., Popot J. L., Sobel A. Some structural properties of the cholinergic receptor protein in its membrane environmental relevant to its function as a pharmacological receptor. Cold Spring Harb Symp Quant Biol. 1976;40:211–230. doi: 10.1101/sqb.1976.040.01.023. [DOI] [PubMed] [Google Scholar]
  6. Cohen M. W. The development of neuromuscular connexions in the presence of D-tubocurarine. Brain Res. 1972 Jun 22;41(2):457–463. doi: 10.1016/0006-8993(72)90515-x. [DOI] [PubMed] [Google Scholar]
  7. Dulhunty A. F., Franzini-Armstrong C. The relative contributions of the folds and caveolae to the surface membrane of frog skeletal muscle fibres at different sarcomere lengths. J Physiol. 1975 Sep;250(3):513–539. doi: 10.1113/jphysiol.1975.sp011068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fischbach G. D., Cohen S. A. The distribution of acetylcholine sensitivity over uninnervated and innervated muscle fibers grown in cell culture. Dev Biol. 1973 Mar;31(1):147–162. doi: 10.1016/0012-1606(73)90326-6. [DOI] [PubMed] [Google Scholar]
  9. Henkart M., Landis D. M., Reese T. S. Similarity of junctions between plasma membranes and endoplasmic reticulum in muscle and neurons. J Cell Biol. 1976 Aug;70(2 Pt 1):338–347. doi: 10.1083/jcb.70.2.338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heuser J. E., Reese T. S., Landis D. M. Functional changes in frog neuromuscular junctions studied with freeze-fracture. J Neurocytol. 1974 Mar;3(1):109–131. doi: 10.1007/BF01111936. [DOI] [PubMed] [Google Scholar]
  11. Hourani B. T., Torain B. F., Henkart M. P., Carter R. L., Marchesi V. T., Fischbach G. D. Acetylcholine receptors of cultured muscle cells demonstrated with ferritin-alpha-bungarotoxin conjugates. J Cell Sci. 1974 Nov;16(2):473–479. doi: 10.1242/jcs.16.2.473. [DOI] [PubMed] [Google Scholar]
  12. Kidokoro Y., Heinemann S., Schubert D., Brandt B. L., Klier F. G. Synapse formation and neurotrophic effects on muscle cell lines. Cold Spring Harb Symp Quant Biol. 1976;40:373–388. doi: 10.1101/sqb.1976.040.01.036. [DOI] [PubMed] [Google Scholar]
  13. Ko P. K., Anderson M. J., Cohen M. W. Denervated skeletal muscle fibers develop discrete patches of high acetylcholine receptor density. Science. 1977 Apr 29;196(4289):540–542. doi: 10.1126/science.850796. [DOI] [PubMed] [Google Scholar]
  14. Nelson P. G. Nerve and muscle cells in culture. Physiol Rev. 1975 Jan;55(1):1–61. doi: 10.1152/physrev.1975.55.1.1. [DOI] [PubMed] [Google Scholar]
  15. Peper K., Dreyer F., Sandri C., Akert K., Moor H. Structure and ultrastructure of the frog motor endplate. A freeze-etching study. Cell Tissue Res. 1974 Jun 24;149(4):437–455. doi: 10.1007/BF00223024. [DOI] [PubMed] [Google Scholar]
  16. Porter C. W., Barnard E. A. The density of cholinergic receptors at the endplate postsynaptic membrane: ultrastructural studies in two mammalian species. J Membr Biol. 1975;20(1-2):31–49. doi: 10.1007/BF01870626. [DOI] [PubMed] [Google Scholar]
  17. Rash J. E., Ellisman M. H. Studies of excitable membranes. I. Macromolecular specializations of the neuromuscular junction and the nonjunctional sarcolemma. J Cell Biol. 1974 Nov;63(2 Pt 1):567–586. doi: 10.1083/jcb.63.2.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Spitzer N. C., Lamborghini J. E. The development of the action potential mechanism of amphibian neurons isolated in culture. Proc Natl Acad Sci U S A. 1976 May;73(5):1641–1645. doi: 10.1073/pnas.73.5.1641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sytkowski A. J., Vogel Z., Nirenberg M. W. Development of acetylcholine receptor clusters on cultured muscle cells. Proc Natl Acad Sci U S A. 1973 Jan;70(1):270–274. doi: 10.1073/pnas.70.1.270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vogel Z., Daniels M. P. Ultrastructure of acetylcholine receptor clusters on cultured muscle fibers. J Cell Biol. 1976 May;69(2):501–507. doi: 10.1083/jcb.69.2.501. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES