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. Author manuscript; available in PMC: 2014 Jul 28.
Published in final edited form as: Circulation. 2012 Aug 24;126(15):1907–1916. doi: 10.1161/CIRCULATIONAHA.112.106245

Figure 2. BMPR2 isoform ratios in CL derived from carriers (light gray, n=35) and affected individuals (dark gray, n=47).

Figure 2

A) Relative isoform-B versus isoform-A (B/A) mRNA ratio as determined by isoform specific real-time PCR. Real time PCR analysis was done three times with each sample run in triplicate. Results from one representative experiment are shown as box-and-whiskers plots with whiskers indicating Tukey whiskers and extreme data points indicated by filled circles. B and C) Western blot validation of the B/A ratios. Total cellular protein extracts from CL cell lines from 9 affected individuals with the highest B/A ratio and 7 carriers with the lowest B/A ratio were analyzed. Isoform mRNA expression data were analyzed in a nonparametric (Mann Whitney U-test) manner while WB densiometry data were analyzed using the Student's t-test. An α-level of 0.05 was chosen, and P-values < 0.05 were considered statistically significant. All P-values were two-tailed.