Skip to main content
. Author manuscript; available in PMC: 2014 Jul 28.
Published in final edited form as: Circulation. 2012 Aug 24;126(15):1907–1916. doi: 10.1161/CIRCULATIONAHA.112.106245

Figure 5. Splicing factor levels in high and low B/A ratio cell lines.

Figure 5

A) SRSF2 levels as detected by western blot analysis and shown as densitometry units in low (light gray, n=5) and high (dark gray, n=6) B/A ratio cell lines. B) SRPK1 levels as detected by western blot analysis and shown as densitometry units in low (light gray, n=5) and high (dark gray, n=6) B/A ratio cell lines. C) hnRNP1 levels as detected by western blot analysis and shown as densitometry units in low (light gray, n=5) and high (dark gray, n=6) B/A ratio cell lines. D) Real time PCR (graph) and western blot (inset) confirmation of siRNA-mediated SRSF2 knockdown in PMVECs. E) Determination of B/A ratio by real-time PCR in PMVECs after siRNA-mediated knockdown of SRSF2. An α-level of 0.05 was chosen, and P-values < 0.05 were considered statistically significant. All P-values were two-tailed.