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. 1978 Feb;75(2):645–649. doi: 10.1073/pnas.75.2.645

An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion.

K Geider, E Beck, H Schaller
PMCID: PMC411312  PMID: 204927

Abstract

Phage fd DNA complexed with DNA binding protein I was used by Escherichia coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) to synthesize an RNA at the origin of single strand to double strand replication. The isolated ori-RNA gave a simple fingerprint after nucleolytic digestion and has a length of about 30 nucleotides. The characterization of the oligonucleotides from the nuclease digest and the extension of the ori-RNA with DNA polymerase I and subsequent restriction of the DNA gave its exact localization in the fd genome, and its total sequence was deduced from the known DNA sequence in this region.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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