Figure 2.

The tripartite Pam₃CysSK₄-containing vaccine generated both cellular and humoral responses. A) Induction of ADCC. Tumor cells (C57mg.MUC1) were labeled with chromium for 2 h and then incubated with serum (1:25 diluted) obtained from mice immunized with IFA, 1 and 2 in liposomes, or 3 and 4 together with IFA for 30 min at 37 °C. The tumor cells were then incubated with effector cells (NK cells, KY-1 clone) at an effector/target ratio of 50:1 for 4 h. Spontaneous release was 14 % of complete release. B) Induction of cytolytic T-cells in MUC1.Tg mice. CD62L^low T-cells—isolated from lymph nodes of mice immunized with IFA, 1 and 2 in liposomes, or 3 and 4 together with IFA and cultured for two weeks with DCs pulsed with glycopeptide SAPDT-(αGalNAc)RPAP for 1–4 or unpulsed for IFA—were subjected to a ⁵¹Cr-release assay with EL4.MUC1 cells as targets. Spontaneous lysis was less than 15 % of total lysis. Each data point represents an individual mouse, and the horizontal lines each indicate the mean for the group of mice. Asterisks indicate statistically significant difference (** P <0.01, *P <0.05), and ns indicates no significant difference.