Abstract
The genesis of plant vacuoles has been studied by high-voltage and conventional electron microscopy. Vacuole genesis is a lysosomal multistep process: (i) GERL (Golgi-associated Endoplasmic Reticulum from which Lysosomes apparently form) produces provacuoles into which lysosomal enzymes and probably other materials appear to be concentrated and packaged; (ii) GERL-derived provacuoles cooperate to drive a programmed cellular autophagy leading to young vacuoles; and (iii) the young vacuoles swell and fuse together into a few large mature vacuoles which continue to collect the GERL-derived provacuoles throughout the life of the cell.
Keywords: electron microscopy, enzyme localizations, lysosome, cellular autophagy, membrane flow
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