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. 1978 Feb;75(2):901–904. doi: 10.1073/pnas.75.2.901

Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.

S E Hutchings, G H Sato
PMCID: PMC411365  PMID: 273251

Abstract

HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation, and a growth rate equal to that of serum-supplemented medium. The omission of any one of the five components results in less than maximal cell growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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