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. 2014 Jul 25;9(7):e104052. doi: 10.1371/journal.pone.0104052

Correction: Evaluation of Monoclonal Antibody-Based Sandwich Direct ELISA (MSD-ELISA) for Antigen Detection of Foot-and-Mouth Disease Virus Using Clinical Samples

The PLOS ONE Staff
PMCID: PMC4114253

Table 1 appears incorrectly due to errors in the typesetting process. The correct version of Table 1 can be viewed below.

Table 1. Comparison of the results of FMDV antigen detection methods using saliva of FMDV-inoculated pigs.

Inoculated Pig Days post-inoculation Inoculated Pig Days post-inoculation
virus no. Methods* 0 1 2 3 4 5 6 virus no. Methods* 0 1 2 3 4 5 6
O/JPN/2000 1 MS - - - + ‖‖ + - - A15 TAI 1/60 1 MS - + + + - - -
SS - - - ++ + + - SS - ++ ++ ++ + - -
IS - - - - - - - IS - + + - - - -
rPCR - - + ++ ++ ++ + rPCR - +++ +++ ++ + + +
2 MS - - - - - + - 2 MS - - +++ + + - -
SS - - - - + ++ - SS - - +++ +++ + - -
IS - - - - - - - IS - - ++ - - - -
rPCR - - - + ++ ++ + rPCR - + +++ +++ ++ + +
3 MS - - - - - - - 3 MS - - + + + - -
SS - - + - - - - SS - + ++ +++ ++ - -
IS - - - - - - - IS - - - + - - -
rPCR - - ++ ++ ++ + + rPCR - ++ ++ +++ ++ + +
4 MS - - - + + - - 4 MS - - - + + - -
SS - - + +++ + - - SS - - + ++ ++ - -
IS - - - - - - - IS - - - - - - -
rPCR - - ++ ++ ++ + + rPCR - + ++ ++ ++ + +
5 MS - - - + - - - 5 MS - + + + - + -
SS - - + + - - - SS - ++ +++ +++ + + -
IS - - - - - - - IS - - + + - - -
rPCR - - + ++ + + + rPCR - ++ +++ +++ ++ ++ +
6 MS - - + - - - - 6 MS - + + - - - -
SS - - +++ + - - - SS - ++ +++ +++ - - -
IS - - + - - - - IS - - + - - - -
rPCR - - ++ ++ + - - rPCR - ++ +++ ++ + + +
O1 BFS1860 1 MS - + ++ + + - - Asia1 Shamir 1 MS - - - + - - -
SS - +++ +++ +++ + - - SS - - + ++ + - -
IS - - - - - + - IS - - - + - - -
rPCR - ++ ++ ++ + + - rPCR - - ++ ++ ++ - -
2 MS - - + + - - - 2 MS - - - + + - -
SS - + +++ ++ - - - SS - - + +++ + - -
IS - - - - - - - IS - - - + - - -
rPCR - ++ ++ ++ + + - rPCR - + ++ ++ ++ + +
3 MS - - +
SS - - +++ §
IS - - -
rPCR - + ++
4 MS - +
SS - +++ §
IS - -
rPCR - +++
5 MS - - ++ +++ - - -
SS - + +++ +++ + - -
IS - + + + - + -
rPCR - +++ ++ ++ + + -
6 MS - - ++ ++
SS - + +++ +++ §
IS - - + -
rPCR - ++ ++ ++
*

MS: MSD-ELISA for multi-serotypes; SS: MSD-ELISA for single serotypes (O, A, Asia1); IS: Indirect sandwich-ELISA for each serotype (O, A, Asia1); rPCR: real-time RT-PCR.

The OD results (average sample OD-average buffer OD) of the MS, SS and IS ELISAs were as +++, >1.0; ++, 0.51.0; +, 0.10.5; and −, < 0.1.

The results-related plaque-forming unit of rPCR were as +++,<104; ++, 102-103; +, 100-102; and −, >100.

§

The pigs inoculated with virus were euthanized.

‖‖

Squares mean the day the obvious vesicular appeared except for the inoculated site.

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