Abstract
The glucose-containing oligosaccharides formed by calf thyroid slices incubated with radioactive glucose were studied. A compound soluble in chloroform/methanol/water, 1:1:0.3 (vol/vol), was found that was indistinguishable from the previously described glucose-containing dolichyl diphosphate oligosaccharide formed by liver microsomes. Glycopeptides were prepared by treating the glycoproteins with pronase, the amino acids were removed with alkaline borohydride, and the products were examined by paper electrophoresis and chromatography. A saccharide equal to that which occurs in the glucose-containing dolichyl diphosphate oligosaccharide could not be detected but glucose was found in oligosaccharides that seemed to be smaller by about three to five monosaccharide residues. The same results were obtained by direct treatment of the glycoproteins with alkaline borohydride.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arima T., Spiro M. J., Spiro R. G. Studies on the carbohydrate units of thyroglobulin. Evaluation of their microheterogeneity in the human and calf proteins. J Biol Chem. 1972 Mar 25;247(6):1825–1835. [PubMed] [Google Scholar]
- Behrens N. H., Parodi A. J., Leloir L. F. Glucose transfer from dolichol monophosphate glucose: the product formed with endogenous microsomal acceptor. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2857–2860. doi: 10.1073/pnas.68.11.2857. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chen W. W., Lennarz W. J., Tarentino A. L., Maley F. A lipid-linked oligosaccharide intermediate in glycoprotein synthesis in oviduct. Structural studies on the oligosaccharide chain. J Biol Chem. 1975 Sep 10;250(17):7006–7013. [PubMed] [Google Scholar]
- Parodi A. J., Behrens N. H., Leloir L. F., Carminatti H. The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3268–3272. doi: 10.1073/pnas.69.11.3268. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parodi A. J., Staneloni R., Cantarella A. I., Leloir L. F., Behrens N. H., Carminatti H., Levy J. A. Further studies on a glycolipid formed from dolichyl-D-glucosyl monophosphate. Carbohydr Res. 1973 Feb;26(2):393–400. doi: 10.1016/s0008-6215(00)84527-9. [DOI] [PubMed] [Google Scholar]
- Parodi A. J. Synthesis of glycosyl-dolichol derivatives in bakers' yeast and their role in protein glycosylation. Eur J Biochem. 1977 May 2;75(1):171–180. doi: 10.1111/j.1432-1033.1977.tb11514.x. [DOI] [PubMed] [Google Scholar]
- Robbins P. W., Krag S. S., Liu T. Effects of UDP-glucose addition on the synthesis of mannosyl lipid-linked oligosaccharides by cell-free fibroblast preparations. J Biol Chem. 1977 Mar 10;252(5):1780–1785. [PubMed] [Google Scholar]
- Spiro M. J., Spiro R. G., Bhoyroo V. D. Lipid-saccharide intermediates in glycoprotein biosynthesis. I. Formation of an oligosaccharide-lipid by thyroid slices and evaluation of its role in protein glycosylation. J Biol Chem. 1976 Oct 25;251(20):6400–6408. [PubMed] [Google Scholar]
- Spiro M. J., Spiro R. G., Bhoyroo V. D. Lipid-saccharide intermediates in glycoprotein biosynthesis. III. Comparison of oligosaccharide-lipids formed by slices from several tissues. J Biol Chem. 1976 Oct 25;251(20):6420–6425. [PubMed] [Google Scholar]
- Spiro R. G., Spiro M. J., Bhoyroo V. D. Lipid-saccharide intermediates in glycoprotein biosynthesis. II. Studies on the structure of an oligosaccharide-lipid from thyroid. J Biol Chem. 1976 Oct 25;251(20):6409–6419. [PubMed] [Google Scholar]
- Waechter C. J., Lennarz W. J. The role of polyprenol-linked sugars in glycoprotein synthesis. Annu Rev Biochem. 1976;45:95–112. doi: 10.1146/annurev.bi.45.070176.000523. [DOI] [PubMed] [Google Scholar]