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Sec incorporation and SBP2 in the nematode lineage. Nematodes from the clades III, IV, and V were examined for the presence of Sec incorporation machinery. Nematodes from the clade IV have lost the ability to incorporate Sec. Nematode SBP2s are the shortest eukaryote SBP2. For comparison D. melanogaster and human SBP2s are included in the phylogenetic analysis. RBDs are represented in light upward diagonal and Sec incorporation domains in wide upward diagonal.
