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. 2014 Jul;20(7):1046–1056. doi: 10.1261/rna.044917.114

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© 2014 Kossinova et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — CTSBP2–ES7L-E cross-linking with diepoxybutane. Modified nucleotides are marked on the right of the gels. The modiﬁcation site is one nucleotide prior to the stop (e.g., the stop observed at A1184 means that C1183 was modified); when the reverse transcription stop was observed at a G, this position was considered as modified because diepoxybutane reacts with the N7G atom which is not involved in Watson–Crick base-pairing: Reverse transcriptase only pauses. (Lane 7) Wild-type CTSBP2. Lane 8 contained the SBP2 mutant version MutSBP2. Lanes 6,7,8 contained diepoxybutane (Deb). Lane 5 lacked Deb. U,G,C,A lanes are sequencing markers. Cross-linked nucleotides (shown in gray circles) were mapped onto the secondary structure of ES7L-E (Anger et al. 2013).