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. 1978 Mar;75(3):1451–1455. doi: 10.1073/pnas.75.3.1451

Transfection of Fv-1 permissive and restrictive mouse cells with integrated DNA of murine leukemia viruses.

I C Hsu, W K Yang, R W Tennant, A Brown
PMCID: PMC411490  PMID: 206900

Abstract

Whole-cell DNA preparations isolated from SC-1 cells chronically infected with N- or B-tropic murine leukemia viruses (MuLV) were tested for infectious activity in an Fv-1n (NIH-3T3) and two Fv-Ib (C57BL/6 and SV-A31) cell cultures. Efficiency of transfection for all DNAs was better in the NIH-3T3 cells than in C57BL/6 or SV-A31 cells; and an [N-tropic MuLV]SC-1 cell DNA preparation was slightly more infectious than a [B-tropic MuLV]SC-1 cell DNA preparation in all three cell cultures, regardless of their Fv-1 genotypes. Progeny viruses from the transfection showed N- or B-tropism corresponding to that of the parent viruses produced by the infected SC-1 cells that were used for the DNA preparation. DNA dose-response studies in NIH-3T3 cells revealed a one-hit mechanism for both the [B-tropic MuLV]SC-1 cell DNA and the [N-tropic MuLV]SC-1 cell DNA preparation. These results demonstrate that, in contrast to virion infection, transfection of N- and B-tropic MuLV with DNA preparations from chronically infected cells is not affected by the Fv-1 gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axelrad A. Genetic control of susceptibility to Friend leukemia virus in mice: studies with the spleen focus assay method. Natl Cancer Inst Monogr. 1966 Sep;22:619–629. [PubMed] [Google Scholar]
  2. Blackstein M. E., Kochman M. A. Inherited resistance to N- and B-tropic murine leukemia viruses in vitro: effect of dexamethasone on the expression of the Fv-1 gene in the congenic strains SIM and SIM.R. Virology. 1976 Oct 1;74(1):252–255. doi: 10.1016/0042-6822(76)90150-1. [DOI] [PubMed] [Google Scholar]
  3. Cooper G. M., Temin H. M. Infectious rous sarcoma virus and reticuloendotheliosis virus DNAs. J Virol. 1974 Nov;14(5):1132–1141. doi: 10.1128/jvi.14.5.1132-1141.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Declève A., Niwa O., Gelmann E., Kaplan H. S. Replication kinetics of N- and B-tropic murine leukemia viruses on permissive and nonpermissive cells in vitro. Virology. 1975 Jun;65(2):320–332. doi: 10.1016/0042-6822(75)90038-0. [DOI] [PubMed] [Google Scholar]
  5. Fenyö E. M., Grundner G., Wiener F., Klein E., Klein G., Harris H. The influence of the partner cell on the production of L virus and the expression of viral surface antigen in hybrid cells. J Exp Med. 1973 May 1;137(5):1240–1255. doi: 10.1084/jem.137.5.1240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fritsch E., Temin H. M. Formation and structure of infectious DNA of spleen necrosis virus. J Virol. 1977 Jan;21(1):119–130. doi: 10.1128/jvi.21.1.119-130.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
  8. Hartley J. W., Rowe W. P., Huebner R. J. Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. J Virol. 1970 Feb;5(2):221–225. doi: 10.1128/jvi.5.2.221-225.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hill M., Hillová J. Production virale dans les fibroblastes de poule traités par l'acide désoxyribonucléique de cellulex XC de rat transformées par le virus de Rous. C R Acad Sci Hebd Seances Acad Sci D. 1971 Jun 14;272(24):3094–3097. [PubMed] [Google Scholar]
  10. Hsu I. C., Yang W. K. DNA transfection of ecotropic murine leukemia viruses in mouse cell cultures. Cancer Res. 1977 Jun;37(6):1709–1714. [PubMed] [Google Scholar]
  11. Huang A. S., Besmer P., Chu L., Baltimore D. Growth of pseudotypes of vesicular stomatitis virus with N-tropic murine leukemia virus coats in cells resistant to N-tropic viruses. J Virol. 1973 Sep;12(3):659–662. doi: 10.1128/jvi.12.3.659-662.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jolicoeur P., Baltimore D. Effect of Fv-1 gene product on proviral DNA formation and integration in cells infected with murine leukemia viruses. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2236–2240. doi: 10.1073/pnas.73.7.2236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jolicoeur P., Baltimore D. Effect of Fv-1 gene product on synthesis of N-tropic and B-tropic murine leukemia viral RNA. Cell. 1976 Jan;7(1):33–39. doi: 10.1016/0092-8674(76)90252-x. [DOI] [PubMed] [Google Scholar]
  14. Karpas A., Milstein C. Recovery of the genome of murine sarcoma virus (MSV) after infection of cells with nuclear DNA from MSV transformed non-virus producing cells. Eur J Cancer. 1973 Apr;9(4):295–299. doi: 10.1016/0014-2964(73)90097-2. [DOI] [PubMed] [Google Scholar]
  15. Krontiris T. G., Soeiro R., Fields B. N. Host restriction of Friend leukemia virus. Role of the viral outer coat. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2549–2553. doi: 10.1073/pnas.70.9.2549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lilly F. Fv-2: identification and location of a second gene governing the spleen focus response to Friend leukemia virus in mice. J Natl Cancer Inst. 1970 Jul;45(1):163–169. [PubMed] [Google Scholar]
  17. Nicolson M. O., Hariri F., Krempin H. M., McAllister R. M., Gilden R. V. Infectious proviral DNA in human cells infected with transformation-defective type C viruses. Virology. 1976 Apr;70(2):301–312. doi: 10.1016/0042-6822(76)90273-7. [DOI] [PubMed] [Google Scholar]
  18. Niwa O., Decléve A., Kaplan H. S. Conversion of restrictive mouse cells to permissiveness during sequential and mixed double infection by murine leukemia viruses. Virology. 1976 Oct 1;74(1):140–153. doi: 10.1016/0042-6822(76)90136-7. [DOI] [PubMed] [Google Scholar]
  19. Odaka T. Inheritance of susceptibility to Friend mouse leukemia virus. V. Introduction of a gene responsible for susceptibility in the genetic complement of resistant mice. J Virol. 1969 Jun;3(6):543–548. doi: 10.1128/jvi.3.6.543-548.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pincus T., Hartley J. W., Rowe W. P. A major genetic locus affecting resistance to infection with murine leukemia viruses. I. Tissue culture studies of naturally occurring viruses. J Exp Med. 1971 Jun 1;133(6):1219–1233. doi: 10.1084/jem.133.6.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pincus T., Hartley J. W., Rowe W. P. A major genetic locus affecting resistance to infection with murine leukemia viruses. IV. Dose-response relationships in Fv-1-sensitive and resistant cell cultures. Virology. 1975 Jun;65(2):333–342. doi: 10.1016/0042-6822(75)90039-2. [DOI] [PubMed] [Google Scholar]
  22. Ray U., Soeiro R., Fields B. N. Host restriction of Friend Leukemia virus coat protein synthesis. J Virol. 1976 Apr;18(1):370–373. doi: 10.1128/jvi.18.1.370-373.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rein A., Kashmiri S. V., Bassin R. H., Gerwin B. L., Duran-Troise G. Phenotypic mixing between N- and B-tropic murine leukemia viruses: infectious particles with dual sensitivity to Fv-1 restriction. Cell. 1976 Mar;7(3):373–379. doi: 10.1016/0092-8674(76)90166-5. [DOI] [PubMed] [Google Scholar]
  24. Rowe W. P., Humphrey J. B., Lilly F. A major genetic locus affecting resistance to infection with murine leukemia viruses. 3. Assignment of the Fv-1 locus to linkage group 8 of the mouse. J Exp Med. 1973 Mar 1;137(3):850–853. doi: 10.1084/jem.137.3.850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  26. Smotkin D., Gianni A. M., Rozenblatt S., Weinberg R. A. Infectious viral DNA of murine leukemia virus. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4910–4913. doi: 10.1073/pnas.72.12.4910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sveda M. M., Fields B. N., Soeiro R. Host restriction of friend leukemia virus; fate of input virion RNA. Cell. 1974 Aug;2(4):271–277. doi: 10.1016/0092-8674(74)90021-x. [DOI] [PubMed] [Google Scholar]
  28. Sveda M. M., Soeiro R. Host restriction of Friend leukemia virus: synthesis and integration of the provirus. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2356–2360. doi: 10.1073/pnas.73.7.2356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Svoboda J., Hlozánek I., Mach O. Detection of chicken sarcoma virus after transfection of chicken fibroblasts with DNA isolated from mammalian cells transformed with Rous Virus. Folia Biol (Praha) 1972;18(2):149–153. [PubMed] [Google Scholar]
  30. TEMIN H. M. THE EFFECTS OF ACTINOMYCIN D ON GROWTH OF ROUS SARCOMA VIRUS IN VITRO. Virology. 1963 Aug;20:577–582. doi: 10.1016/0042-6822(63)90282-4. [DOI] [PubMed] [Google Scholar]
  31. Tennant R. W., Myer F. E., McGrath L. Effect of the Fv-1 gene on leukemia virus in mouse cell heterokaryons. Int J Cancer. 1974 Oct 15;14(4):504–513. doi: 10.1002/ijc.2910140410. [DOI] [PubMed] [Google Scholar]
  32. Tennant R. W., Schluter B., Yang W., Brown A. Reciprocal inhibition of mouse leukemia virus infection by Fv-1 allele cell extracts. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4241–4245. doi: 10.1073/pnas.71.10.4241. [DOI] [PMC free article] [PubMed] [Google Scholar]

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