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. 1978 Mar;75(3):1505–1509. doi: 10.1073/pnas.75.3.1505

Pseudotypes of feline sarcoma virus contain an 85,000-dalton protein with feline oncornavirus-associated cell membrane antigen (FOCMA) activity.

C J Sherr, A Sen, G J Todaro, A Sliski, M Essex
PMCID: PMC411501  PMID: 206902

Abstract

Feline sarcoma virus (FeSV) rescued from transformed nonproducer mink or rat cells contains two FeSV-specific antigens (p15 and p12), and the feline oncornavirus-associated cell membrane antigen (FOCMA). All three antigens are helper virus-independent and are encoded by the FeSV genome, FOCMA, p15, and p12 antigens cochromatograph as phosphorylated molecules of 85,000 molecular weight (pp85), adsorb to immunoadsorbant columns prepared with antibodies to feline leukemia virus (FeLV), and are precipitated with antisera to FeLV or FOCMA. Antibodies to FOCMA can be adsorbed with fractions containing pp85 but not with FeLV proteins, including p15 and p12. Thus, a virus-coded tumor antigen which immunizes cats against tumors induced by feline type C viruses is packaged in FeSV particles and is linked to viral structural protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbacid M., Stephenson J. R., Aaronson S. A. Evolutionary relationships between gag gene-coded proteins of murine and primate endogenous type C RNA viruses. Cell. 1977 Apr;10(4):641–648. doi: 10.1016/0092-8674(77)90097-6. [DOI] [PubMed] [Google Scholar]
  2. Benveniste R. E., Lieber M. M., Livingston D. M., Sherr C. J., Todaro G. J., Kalter S. S. Infectious C-type virus isolated from a baboon placenta. Nature. 1974 Mar 1;248(5443):17–20. doi: 10.1038/248017a0. [DOI] [PubMed] [Google Scholar]
  3. Essex M. Immunity to leukemia, lymphoma, and fibrosarcoma in cats: a case for immunosurveillance. Contemp Top Immunobiol. 1977;6:71–106. doi: 10.1007/978-1-4684-3051-6_2. [DOI] [PubMed] [Google Scholar]
  4. Essex M., Jakowski R. M., Hardy W. D., Jr, Cotter S. M., Hess P., Sliski A. Feline oncornavirus-associated cell membrane antigen. III. Antibody titers in cats from leukemia cluster households. J Natl Cancer Inst. 1975 Mar;54(3):637–641. [PubMed] [Google Scholar]
  5. Essex M., Klein G., Snyder S. P., Harrold J. B. Correlation between humoral antibody and regression of tumours induced by feline sarcoma virus. Nature. 1971 Sep 17;233(5316):195–196. doi: 10.1038/233195a0. [DOI] [PubMed] [Google Scholar]
  6. Essex M., Sliski A., Hardy W. D., Jr, Cotter S. M. Immune response to leukemia virus and tumor-associated antigens in cats. Cancer Res. 1976 Feb;36(2 Pt 2):640–645. [PubMed] [Google Scholar]
  7. Essex M., Snyder S. P. Feline oncornavirus-associated cell membrane antigen. I. Serologic studies with kittens exposed to cell-free materials from various feline fibrosarcomas. J Natl Cancer Inst. 1973 Sep;51(3):1007–1012. doi: 10.1093/jnci/51.3.1007. [DOI] [PubMed] [Google Scholar]
  8. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Giard D. J., Aaronson S. A., Todaro G. J., Arnstein P., Kersey J. H., Dosik H., Parks W. P. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst. 1973 Nov;51(5):1417–1423. doi: 10.1093/jnci/51.5.1417. [DOI] [PubMed] [Google Scholar]
  10. Graves D. C., Velicer L. F. Properties of feline leukemia virus. I. Chromatographic separation and analysis of the polypeptides. J Virol. 1974 Aug;14(2):349–365. doi: 10.1128/jvi.14.2.349-365.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hardy W. D., Jr, Zuckerman E. E., MacEwen E. G., Hayes A. A., Essex M. A feline leukaemia virus- and sarcoma virus-induced tumour-specific antigen. Nature. 1977 Nov 17;270(5634):249–251. doi: 10.1038/270249a0. [DOI] [PubMed] [Google Scholar]
  12. Henderson I. C., Lieber M. M., Todaro G. J. Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses. Virology. 1974 Jul;60(1):282–287. doi: 10.1016/0042-6822(74)90386-9. [DOI] [PubMed] [Google Scholar]
  13. Huebner R. J., Hartley J. W., Rowe W. P., Lane W. T., Capps W. I. Rescue of the defective genome of Moloney sarcoma virus from a noninfectious hamster tumor and the production of pseudotype sarcoma viruses with various murine leukemia viruses. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1164–1169. doi: 10.1073/pnas.56.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Huu Duc-Nguyen, Rosenblum E. N., Zeigel R. F. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J Bacteriol. 1966 Oct;92(4):1133–1140. doi: 10.1128/jb.92.4.1133-1140.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jarrett W., Jarrett O., Mackey L., Laird H., Hood C., Hay D. Vaccination against feline leukaemia virus using a cell membrane antigen system. Int J Cancer. 1975 Jul 15;16(1):134–141. doi: 10.1002/ijc.2910160115. [DOI] [PubMed] [Google Scholar]
  16. Khan A. S., Stephenson J. R. Feline leukemia virus: biochemical and immunological characterization of gag gene-coded structural proteins. J Virol. 1977 Sep;23(3):599–607. doi: 10.1128/jvi.23.3.599-607.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. McAllister R. M., Nicolson M., Gardner M. B., Rongey R. W., Rasheed S., Sarma P. S., Huebner R. J., Hatanaka M., Oroszlan S., Gilden R. V. C-type virus released from cultured human rhabdomyosarcoma cells. Nat New Biol. 1972 Jan 5;235(53):3–6. doi: 10.1038/newbio235003a0. [DOI] [PubMed] [Google Scholar]
  19. Nowinski R. C., Fleissner E., Sarkar N. H., Aoki T. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. II. Mammalian leukemia-sarcoma viruses. J Virol. 1972 Feb;9(2):359–366. doi: 10.1128/jvi.9.2.359-366.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Okasinski G. F., Velicer L. F. Analysis of intracellular feline leukemia virus proteins II. Generation of feline leukemia virus structural proteins from precursor polypeptides. J Virol. 1977 Apr;22(1):74–85. doi: 10.1128/jvi.22.1.74-85.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Olsen R. G., Hoover E. A., Mathes L. E., Heding L., Schaller J. P. Immunization against feline oncarnavirus disease using a killed tumor cell vaccine. Cancer Res. 1976 Oct;36(10):3642–3646. [PubMed] [Google Scholar]
  22. Pal B. K., McAllister R. M., Gardner M. B., Roy-Burman P. Comparative studies on the structural phosphoproteins of mammalian type C viruses. J Virol. 1975 Jul;16(1):123–131. doi: 10.1128/jvi.16.1.123-131.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rickard C. G., Post J. E., Noronha F., Barr L. M. A transmissible virus-induced lymphocytic leukemia of the cat. J Natl Cancer Inst. 1969 Jun;42(6):987–1014. [PubMed] [Google Scholar]
  24. Scolnick E. M., Parks W. P., Livingston D. M. Radioimmunoassay of mammalian type C viral proteins. I. Species specific reactions of murine and feline viruses. J Immunol. 1972 Sep;109(3):570–577. [PubMed] [Google Scholar]
  25. Sen A., Sherr C. J., Todaro G. J. Phosphorylation of murine type C viral p12 proteins regulates their extent of binding to the homologous viral RNA. Cell. 1977 Mar;10(3):489–496. doi: 10.1016/0092-8674(77)90036-8. [DOI] [PubMed] [Google Scholar]
  26. Sen A., Sherr C. J., Todaro G. J. Specific binding of the type C viral core protein p12 with purified viral RNA. Cell. 1976 Jan;7(1):21–32. doi: 10.1016/0092-8674(76)90251-8. [DOI] [PubMed] [Google Scholar]
  27. Sherr C. J., Fedele L. A., Benveniste R. E., Todaro G. J. Interspecies antigenic determinants of the reverse transcriptases and p30 proteins of mammalian type C viruses. J Virol. 1975 Jun;15(6):1440–1448. doi: 10.1128/jvi.15.6.1440-1448.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sherr C. J., Todaro G. J. Radioimmunoassay of the major group specific protein of endogenous baboon type C viruses: relation to the RD-114-CCC group and detection of antigen in normal baboon tissues. Virology. 1974 Sep;61(1):168–181. doi: 10.1016/0042-6822(74)90252-9. [DOI] [PubMed] [Google Scholar]
  29. Sliski A. H., Essex M., Meyer C., Todaro G. Feline oncornavirus-associated cell membrane antigen: expression in transformed nonproducer mink cells. Science. 1977 Jun 17;196(4296):1336–1339. doi: 10.1126/science.194310. [DOI] [PubMed] [Google Scholar]
  30. Stephenson J. R., Essex M., Hino S., Hardy W. D., Jr, Aaronson S. A. Feline oncornavirus-associated cell-membrane antigen (FOCMA): distinction between FOCMA and the major virion glycoprotein. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1219–1223. doi: 10.1073/pnas.74.3.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Stephenson J. R., Khan A. S., Sliski A. H., Essex M. Feline oncornavirus-associated cell membrane antigen: evidence for an immunologically crossreactive feline sarcoma virus-coded protein. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5608–5612. doi: 10.1073/pnas.74.12.5608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Theilen G. H., Gould D., Fowler M., Dungworth D. L. C-type virus in tumor tissue of a woolly monkey (Lagothrix spp.) with fibrosarcoma. J Natl Cancer Inst. 1971 Oct;47(4):881–889. [PubMed] [Google Scholar]
  33. Todaro G. J., Arnstein P., Parks W. P., Lennette E. H., Huebner R. J. A type-C virus in human rhabdomyosarcoma cells after inoculation into NIH Swiss mice treated with antithymocyte serum. Proc Natl Acad Sci U S A. 1973 Mar;70(3):859–862. doi: 10.1073/pnas.70.3.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Todaro G. J., Sherr C. J., Benveniste R. E., Lieber M. M., Melnick J. L. Type C viruses of baboons: isolation from normal cell cultures. Cell. 1974 May;2(1):55–61. doi: 10.1016/0092-8674(74)90008-7. [DOI] [PubMed] [Google Scholar]

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