Abstract
Fusion between P3 x 63 Ag8 mouse myeloma cells and spleen cells from BALB/c mice immunized with influenza type A or B or parainfluenza type 1 virus generated reproducibly antiviral antibody-producing somatic cell hybrids (hybridomas). Eleven hybridomas derived from spleen cells of mice immunized with influenza type A virus were directed against the viral hemagglutinin, one reacted with a host component derived from chickens, and one expressed a specificity not further characterized. The hybridoma antibodies tended to be highly specific for the hemagglutinin of the immunizing virus and seemed to express the same repertoire of strain-specific antibody reactivities as splenic precursor B cells, they did not express any of the frequently occurring crossreactive anti-hemagglutinin specificities. Hybridomas producing crossreactive antibodies against hemagglutinin could be obtained if priming and boosting virus were heterologous.
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Selected References
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- Cancro M. P., Sigal N. H., Klinman N. R. Differential expression of an equivalent clonotype among BALB/c and C57BL/6 mice. J Exp Med. 1978 Jan 1;147(1):1–12. doi: 10.1084/jem.147.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coffino P., Scharff M. D. Rate of somatic mutation in immunoglobulin production by mouse myeloma cells. Proc Natl Acad Sci U S A. 1971 Jan;68(1):219–223. doi: 10.1073/pnas.68.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Croce C. M., Koprowski H., Eagle H. Effect of environmental pH on the efficiency of cellular hybridization. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1953–1956. doi: 10.1073/pnas.69.7.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
- Gerhard W., Braciale T. J., Klinman N. R. The analysis of the monoclonal immune response to influenza virus. I. Production of monoclonal anti-viral antibodies in vitro. Eur J Immunol. 1975 Oct;5(10):720–725. doi: 10.1002/eji.1830051013. [DOI] [PubMed] [Google Scholar]
- Gerhard W. The analysis of the monoclonal immune response to influenza virus. II. The antigenicity of the viral hemagglutinin. J Exp Med. 1976 Oct 1;144(4):985–995. doi: 10.1084/jem.144.4.985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kilbourne E. D. Future influenza vaccines and the use of genetic recombinants. Bull World Health Organ. 1969;41(3):643–645. [PMC free article] [PubMed] [Google Scholar]
- Koprowski H., Gerhard W., Croce C. M. Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985–2988. doi: 10.1073/pnas.74.7.2985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Köhler G., Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol. 1976 Jul;6(7):511–519. doi: 10.1002/eji.1830060713. [DOI] [PubMed] [Google Scholar]
- LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
- Spring S. B., Nisonoff A. Allotypic markers on Fab fragments of mouse immunoglobulins. J Immunol. 1974 Aug;113(2):470–478. [PubMed] [Google Scholar]
- Steiner L. A., Blumberg P. M. Mercury derivatives of the Fab and Fc fragments of a human myeloma protein. Biochemistry. 1971 Dec 7;10(25):4725–4739. doi: 10.1021/bi00801a020. [DOI] [PubMed] [Google Scholar]