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. 1979 Sep;76(9):4235–4239. doi: 10.1073/pnas.76.9.4235

Identification of mannose 6-phosphate in glycoproteins that inhibit the assimilation of β-galactosidase by fibroblasts

Jack Distler 1,2,3, Virginia Hieber 1,2,3, Gary Sahagian 1,2,3, Roy Schmickel 1,2,3, George W Jourdian 1,2,3
PMCID: PMC411547  PMID: 116230

Abstract

Bovine testicular β-galactosidase (β-D-galactoside galactohydrolase, EC 3.2.1.23) is rapidly and selectively assimilated by human skin fibroblasts. The assimilation of the enzyme is strongly inhibited by mannose 6-phosphate and by a glycoprotein fraction isolated from bovine testes (glycoprotein inhibitors). These results suggest that β-galactosidase and the glycoprotein inhibitors have a common recognition marker that contains mannose 6-phosphate. The presence of mannose phosphate in the glycoprotein inhibitors was demonstrated by acid hydrolysis of the glycoproteins to liberate mannose phosphate followed by reduction with NaB3H4 to give [3H]mannitol phosphate. The 3H-labeled compound was identified by paper electrophoresis and by the release of [3H]mannitol on treatment with phosphatase. The [3H]mannitol phosphate was oxidized with periodate and the resulting phosphorylated fragment, on reduction with NaB3H4, yielded [3H]ethylene glycol phosphate, indicating substitution of phosphate on carbon 6 of mannitol. Mannose 6-phosphate was also found in a major carbohydrate-containing fraction of peptides produced from the glycoprotein inhibitors by tryspin digestion. It was estimated that about 2% of the mannose residues were present as mannose 6-phosphate. Phosphorylated oligosaccharides were also identified in hydrolysates of the glycoprotein inhibitors. One, a disaccharide, was identified as α-(mannosyl-6-phosphate)-(1 → 2)-mannose. These observations suggest that the recognition marker of β-galactosidase contains α1,2-linked mannose 6-phosphate; terminal α1,2-linked mannose residues are known to occur in the high-mannose type oligosaccharides present on β-galactosidase.

Keywords: adsorptive endocytosis, recognition marker, lysosomal enzymes

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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