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. 1979 Sep;76(9):4360–4364. doi: 10.1073/pnas.76.9.4360

Cloning of the structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12.

U Henning, H D Royer, R M Teather, I Hindennach, C P Hollenberg
PMCID: PMC411574  PMID: 159455

Abstract

The gene (ompA) for the major outer membrane protein II* from Escherichia coli K-12 has been cloned on a 5-megadalton EcoRI fragment by using phage lambda as vector. The gene is expressed during the lytic cycle of the recombinant phage and the insoluble membrane-bound protein was detected in phage plaques with a simple radioimmunoassay. Transfer of the EcoRI fragment into plasmid pSC101 and expression in a host lacking protein II* led to overproduction of protein II* and decreased production of two other major outer membrane proteins. Expression of the plasmid pSC101-ompA+ in minicells derived from an ompA minicell-producing strain led to synthesis, at high rates, of this protein and massive accumulation of a second cell envelope protein most likely representing the biosynthetic precursor of protein II*.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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