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. 1979 Sep;76(9):4414–4416. doi: 10.1073/pnas.76.9.4414

Cytoplasmic phase separation in formation of galactosemic cataract in lenses of young rats

Coe Ishimoto *, Patrick W Goalwin †, Shao-Tang Sun †, Izumi Nishio †, Toyoichi Tanaka †,‡
PMCID: PMC411585  PMID: 16592709

Abstract

We have determined the age dependence of the characteristics of the cytoplasmic phase separation of lenses from normal and galactosemic young rats. In the normal lens, the temperature at which the phase separation occurs decreases monotonically with age. In the lenses of rats fed with a high galactose diet, the phase separation temperature becomes increasingly higher with the development of galactosemia. When the phase separation temperature becomes higher than the ocular temperature, the nuclear opacity appears in vivo. The opacity is the result of light scattering by spatial fluctuations of the refractive index formed by interspersed regions of two separated phases in the fiber cell cytoplasm. This shows that the nuclear opacity that develops in the lens of galactosemic rats is the manifestation of phase separation of the lens fiber cytoplasm.

Keywords: galactosemia

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Kinoshita J. H. Mechanisms initiating cataract formation. Proctor Lecture. Invest Ophthalmol. 1974 Oct;13(10):713–724. [PubMed] [Google Scholar]
  2. Kinsey V. E., Hightower K. R. Studies on the crystalline lens. XXVII. Kinetic and bioelectric measurements of galactose cataracts in rats. Exp Eye Res. 1978 May;26(5):521–528. doi: 10.1016/0014-4835(78)90061-1. [DOI] [PubMed] [Google Scholar]
  3. Philipson B. Distribution of protein within the normal rat lens. Invest Ophthalmol. 1969 Jun;8(3):258–270. [PubMed] [Google Scholar]
  4. Philipson B. Galactose cataract: changes in protein distribution during development. Invest Ophthalmol. 1969 Jun;8(3):281–289. [PubMed] [Google Scholar]
  5. Sippel T. O. Changes in the water, protein, and glutathione contents of the lens in the course of galactose cataract development in rats. Invest Ophthalmol. 1966 Dec;5(6):568–575. [PubMed] [Google Scholar]
  6. Tanaka T., Ishimoto C., Chylack L. T., Jr Phase separation of a protein-water mixture in cold cataract in the young rat lens. Science. 1977 Sep 2;197(4307):1010–1012. doi: 10.1126/science.887936. [DOI] [PubMed] [Google Scholar]
  7. Tanaka T., Ishimoto C. In vivo observation of protein diffusivity in rabbit lenses. Invest Ophthalmol Vis Sci. 1977 Feb;16(2):135–140. [PubMed] [Google Scholar]
  8. Van Heyningen R. Galactose cataract: a review. Exp Eye Res. 1971 May;11(3):415–428. doi: 10.1016/s0014-4835(71)80054-4. [DOI] [PubMed] [Google Scholar]
  9. ZIGMAN S., LERMAN S. A COLD PRECIPITABLE PROTEIN IN THE LENS. Nature. 1964 Aug 8;203:662–663. doi: 10.1038/203662a0. [DOI] [PubMed] [Google Scholar]

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