Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Sep;76(9):4441–4445. doi: 10.1073/pnas.76.9.4441

DNA synthesis in temperature-sensitive mutants of the cell cycle infected by polyoma virus and adenovirus.

M Rossini, R Weinmann, R Baserga
PMCID: PMC411592  PMID: 228298

Abstract

tsAF8 cells are a temperature-sensitive (ts) mutant of BHK cells that are arrested in G1 at the nonpermissive temperature. When made quiescent by serum restriction, they can be stimulated to enter S phase by 10% serum at 34 degrees C but not at 40.6 degrees C. The same results can be obtained if quiescent cells are infected with polyoma virus or adenovirus 12 instead of serum. However, adenovirus 2 infection stimulates DNA synthesis in tsAF8 cells at both 34 degrees C and 40.6 degrees C. The DNA synthesized after adenovirus 2 infection has been shown to be cellular DNA by CsCl density centrifugation. By density labeling it can be shown that adenovirus 2-induced DNA synthesis is due to semiconservative replication. The difference between adenovirus 2 and polyoma (or serum) is also evident with another ts mutant of BHK cells, ts13 cells. These results open the possibility of identifying the viral or cellular mechanism at the basis of this difference in the induction of host DNA synthesis between adenovirus 2 and polyoma or serum.

Full text

PDF
4441

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashihara T., Chang S. D., Baserga R. Constancy of the shift-up point in two temperature-sensitive mammalian cell lines that arrest in G1. J Cell Physiol. 1978 Jul;96(1):15–22. doi: 10.1002/jcp.1040960103. [DOI] [PubMed] [Google Scholar]
  2. Berk A. J., Sharp P. A. Ultraviolet mapping of the adenovirus 2 early promoters. Cell. 1977 Sep;12(1):45–55. doi: 10.1016/0092-8674(77)90184-2. [DOI] [PubMed] [Google Scholar]
  3. Burstin S. J., Meiss H. K., Basilico C. A temperature-sensitive cell cycle mutant of the BHK cell line. J Cell Physiol. 1974 Dec;84(3):397–408. doi: 10.1002/jcp.1040840308. [DOI] [PubMed] [Google Scholar]
  4. Cooper P. K., Hanawalt P. C. Heterogeneity of patch size in repair replicated DNA in Escherichia coli. J Mol Biol. 1972 Jun 14;67(1):1–10. doi: 10.1016/0022-2836(72)90381-6. [DOI] [PubMed] [Google Scholar]
  5. DULBECCO R., HARTWELL L. H., VOGT M. INDUCTION OF CELLULAR DNA SYNTHESIS BY POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965 Feb;53:403–410. doi: 10.1073/pnas.53.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doerfler W. Nonproductive infection of baby hamster kidney cells (BHK21) with adenovirus type 12. Virology. 1969 Aug;38(4):587–606. doi: 10.1016/0042-6822(69)90179-2. [DOI] [PubMed] [Google Scholar]
  7. Dubbs D. R., Kit S. Reactivation of chick erythrocyte nuclei in heterokaryons with temperature-sensitive Chinese hamster cells. Somatic Cell Genet. 1976 Jan;2(1):11–19. doi: 10.1007/BF01539238. [DOI] [PubMed] [Google Scholar]
  8. Floros J., Ashihara T., Baserga R. Characterization of ts13 cells a temperature-sensitive mutant of the G1 phase of the cell cycle. Cell Biol Int Rep. 1978 May;2(3):259–269. doi: 10.1016/0309-1651(78)90006-1. [DOI] [PubMed] [Google Scholar]
  9. GREEN M., PINA M. BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, VI. PROPERTIES OF HIGHLY PURIFIED TUMORIGENIC HUMAN ADENOVIRUSES AND THEIR DNA. Proc Natl Acad Sci U S A. 1964 Jun;51:1251–1259. doi: 10.1073/pnas.51.6.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laughlin C., Strohl W. A. Factors regulating cellular DNA synthesis induced by adenovirus infection. I. The effects of actinomycin D on G1-arrested BHK21 cells abortively infected with type 12 adenovirus of stimulated by serum. Virology. 1976 Oct 1;74(1):30–43. doi: 10.1016/0042-6822(76)90125-2. [DOI] [PubMed] [Google Scholar]
  11. Laughlin C., Strohl W. A. Factors regulating cellular DNA synthesis induced by adenovirus infection. II. The effects of actinomycin D on productive virus-cell systems. Virology. 1976 Oct 1;74(1):44–56. doi: 10.1016/0042-6822(76)90126-4. [DOI] [PubMed] [Google Scholar]
  12. Levinson A., Levine A. J. The isolation and identification of the adenovirus group C tumor antigens. Virology. 1977 Jan;76(1):1–11. doi: 10.1016/0042-6822(77)90275-6. [DOI] [PubMed] [Google Scholar]
  13. Littlefield J. W., Basilico C. Infection of thymidine kinase-deficient BHK cells with polyoma virus. Nature. 1966 Jul 16;211(5046):250–252. doi: 10.1038/211250a0. [DOI] [PubMed] [Google Scholar]
  14. Nature of temperature-sensitive mutants. J Cell Physiol. 1978 Jun;95(3):365–366. [PubMed] [Google Scholar]
  15. Nishimoto T., Okubo C. K., Raskas H. J. Abortive replication of adenovirus 2 in the temperature-sensitive hamster cell mutant tsAF8. Virology. 1977 May 1;78(1):1–10. doi: 10.1016/0042-6822(77)90074-5. [DOI] [PubMed] [Google Scholar]
  16. Nishimoto T., Raskas H. J., Basilico C. Temperature-sensitive cell mutations that inhibit adenovirus 2 replication. Proc Natl Acad Sci U S A. 1975 Jan;72(1):328–332. doi: 10.1073/pnas.72.1.328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. POPE J. H., ROWE W. P. DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964 Aug 1;120:121–128. doi: 10.1084/jem.120.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pardee A. B., Dubrow R., Hamlin J. L., Kletzien R. F. Animal cell cycle. Annu Rev Biochem. 1978;47:715–750. doi: 10.1146/annurev.bi.47.070178.003435. [DOI] [PubMed] [Google Scholar]
  19. Philipson L., Lonberg-Holm K., Pettersson U. Virus-receptor interaction in an adenovirus system. J Virol. 1968 Oct;2(10):1064–1075. doi: 10.1128/jvi.2.10.1064-1075.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rossini M., Baserga R. RNA synthesis in a cell cycle-specific temperature sensitive mutant from a hamster cell line. Biochemistry. 1978 Mar 7;17(5):858–863. doi: 10.1021/bi00598a017. [DOI] [PubMed] [Google Scholar]
  21. Sauer G., Defendi V. Stimulation of DNA synthesis and complement-fixing antigen production by SV40 in human diploid cell cultures: evidence for "abortive" infection. Proc Natl Acad Sci U S A. 1966 Aug;56(2):452–457. doi: 10.1073/pnas.56.2.452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schlegel R., Benjamin T. L. Cellular alterations dependent upon the polyoma virus Hr-t function: separation of mitogenic from transforming capacities. Cell. 1978 Jul;14(3):587–599. doi: 10.1016/0092-8674(78)90244-1. [DOI] [PubMed] [Google Scholar]
  23. Scott W. A., Brockman W. W., Nathans D. Biological activities of deletion mutants of simian virus 40. Virology. 1976 Dec;75(2):319–334. doi: 10.1016/0042-6822(76)90031-3. [DOI] [PubMed] [Google Scholar]
  24. Sheinin Polyoma and cell DNA synthesis in mouse L cells temperature sensitive for the replication of cell DNA. J Virol. 1976 Mar;17(3):692–704. doi: 10.1128/jvi.17.3.692-704.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Strniste G. F., Rall S. C. Induction of stable protein-deoxyribonucleic acid adducts in Chinese hamster cell chromatin by ultraviolet light. Biochemistry. 1976 Apr 20;15(8):1712–1719. doi: 10.1021/bi00653a019. [DOI] [PubMed] [Google Scholar]
  26. Talavera A., Basilico C. Temperature sensitive mutants of BHK cells affected in cell cycle progression. J Cell Physiol. 1977 Sep;92(3):425–436. doi: 10.1002/jcp.1040920310. [DOI] [PubMed] [Google Scholar]
  27. Taylor-Papadimitriou J., Stoker M. G., Riddle P. Further manifestations of abortive transformation of BHK 21 cells by polyoma virus. Int J Cancer. 1971 Mar 15;7(2):269–276. doi: 10.1002/ijc.2910070211. [DOI] [PubMed] [Google Scholar]
  28. Tjian R., Fey G., Graessmann A. Biological activity of purified simian virus 40 T antigen proteins. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1279–1283. doi: 10.1073/pnas.75.3.1279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Weil R., Salomon E., May E., May P. A simplifying concept in tumor virology: virus-specific "pleiotropic effectors". Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):381–395. doi: 10.1101/sqb.1974.039.01.050. [DOI] [PubMed] [Google Scholar]
  30. Zimmerman J. E., Jr, Raska K., Jr Inhibition of adenovirus type 12 induced DNA synthesis in G1-arrested BHK21 cells by dibutyryl adenosine cyclic 3':5'-monophosphate. Nat New Biol. 1972 Oct 4;239(92):145–147. doi: 10.1038/newbio239145a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES