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. 2014 May 22;5(11):3836–3848. doi: 10.18632/oncotarget.1657

Figure 3. C96 inhibits AKT but not IGF-1R, ERK or c-Src kinase activation.

Figure 3

(A) LP1 and JJN3 cells were starved overnight, then treated with 100 (μM of C96 or S14161 for 2 hrs, followed by IGF-1 (100 ng/mL) for 15 min. After incubation, cells were harvested and the expression of p-AKT, p-IGF-1R, total AKT (T-AKT), and IGF-1R proteins were detected by immunoblotting. (B) LP1 and JJN3 were treated with C96 at indicated concentrations for 24 hrs followed by immunoblotting assay for the expression of p-Src, c-Src, p-ERK, and ERK. GAPDH was used as a loading control.