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. Author manuscript; available in PMC: 2014 Jul 31.
Published in final edited form as: Traffic. 2011 Apr 21;12(7):902–911. doi: 10.1111/j.1600-0854.2011.01194.x

Figure 5. Rab7 interacts with RILP in hCTL.

Figure 5

A) GST and GST-tagged RILP were incubated with hCTL cell lysate pretreated with 2 mm GTPγS, subjected to WB and probed with an anti-Rab7 antibody (Sigma) to detect the endogenous protein. B) hCTL transduced with GFP-RILP stained with anti-Rab7. C) Western blot of lysates from hCTL transduced with GFP-wt RILP (GFP-wt RILP) mock-treated (mock) or nucleofected with 3 μg of non-targeting (NT RNA) or RNA duplexes against human Rab7 (RNA Rab7 #4) as indicated, probed with anti-Rab7 and anti-RILP antibodies, using actin as a loading control. D) Killing assay showing the percentage lysis of targets at different ratios of effector CTL (E) to target P815 (T). Graph shown is representative of two independent experiments, each performed in triplicate. Standard deviations of triplicates were less than 5%. Scale bar: 3 μm.