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. 1979 Nov;76(11):5445–5449. doi: 10.1073/pnas.76.11.5445

Rapid ATP assays in perfused mouse liver by 31P NMR.

A C McLaughlin, H Takeda, B Chance
PMCID: PMC411665  PMID: 293654

Abstract

31P NMR was used to continuously monitor ATP and inorganic phosphate levels in perfused mouse liver. Under "optimal" conditions, the time resolution of the technique was approximately 1 min. In the absence of any metabolic perturbations the ATP level remained constant for at least 2 hr and decreased by only approximately 20% in 18 hr. Both ATP and inorganic phosphate levels responded to alterations in the oxygen supply to the liver. The half-time for this response was approximately 1 min, and the response to short periods of hypoxia or ischemia was partially reversible. The addition of insulin caused only a minor decrease in the ATP level but significantly decreased the rate of response of ATP and phosphate levels to hypoxia and ischemia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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