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. 1979 Nov;76(11):5567–5571. doi: 10.1073/pnas.76.11.5567

Spliced adenovirus-associated virus RNA.

C A Laughlin, H Westphal, B J Carter
PMCID: PMC411690  PMID: 230481

Abstract

We describe the structure of cytoplasmic RNA species transcribed from the DNA of adenovirus-associated virus, a defective parvovirus. The RNA was hybridized with minus strand template DNA and visualized in the electron microscope. Alternatively, the DNA.RNA duplex molecules were digested with nuclease S1 or Escherichia coli exonuclease VII and analyzed by agarose gel electrophoresis. A set of RNA species was observed with 5' terminal at map positions 5, 13, 19, or 39 and a 3' terminus and poly(A) tail at position 96 (one map unit is equivalent to 1% of genome length). Most of these RNAs are spliced and lack sequences approximately between positions 40 and 49. Some RNA preparations also contained unspliced molecules with 5' and 3' terminal at positions similar to those in the spliced RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y., Dhar R., Laub O., Horowitz M., Khoury G. Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3686–3690. doi: 10.1073/pnas.74.9.3686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berget S. M., Moore C., Sharp P. A. Spliced segments at the 5' terminus of adenovirus 2 late mRNA. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171–3175. doi: 10.1073/pnas.74.8.3171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berk A. J., Sharp P. A. Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274–1278. doi: 10.1073/pnas.75.3.1274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berns K. I., Kelly T. J., Jr Letter: Visualization of the inverted terminal repetition in adeno-associated virus DNA. J Mol Biol. 1974 Jan 15;82(2):267–271. doi: 10.1016/0022-2836(74)90344-1. [DOI] [PubMed] [Google Scholar]
  5. Breathnach R., Mandel J. L., Chambon P. Ovalbumin gene is split in chicken DNA. Nature. 1977 Nov 24;270(5635):314–319. doi: 10.1038/270314a0. [DOI] [PubMed] [Google Scholar]
  6. Carter B. J., Khoury G., Denhardt D. T. Physical map and strand polarity of specific fragments of adenovirus-associated virus DNA produced by endonuclease R-EcoRI. J Virol. 1975 Sep;16(3):559–568. doi: 10.1128/jvi.16.3.559-568.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carter B. J., Khoury G. Specific cleavage of adenovirus-associated virus DNA by restriction endonuclease R-EcoRI--characterization of cleavage products. Virology. 1975 Feb;63(2):523–538. doi: 10.1016/0042-6822(75)90325-6. [DOI] [PubMed] [Google Scholar]
  8. Chow L. T., Gelinas R. E., Broker T. R., Roberts R. J. An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA. Cell. 1977 Sep;12(1):1–8. doi: 10.1016/0092-8674(77)90180-5. [DOI] [PubMed] [Google Scholar]
  9. Jay F. T., de la Maza L. M., Carter B. J. Parvovirus RNA transcripts containing sequences not present in mature mRNA: a method for isolation of putative mRNA precursor sequences. Proc Natl Acad Sci U S A. 1979 Feb;76(2):625–629. doi: 10.1073/pnas.76.2.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kitchingman G. R., Lai S. P., Westphal H. Loop structures in hybrids of early RNA and the separated strands of adenovirus DNA. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4392–4395. doi: 10.1073/pnas.74.10.4392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Koczot F. J., Carter B. J., Garon C. F., Rose J. A. Self-complementarity of terminal sequences within plus or minus strands of adenovirus-associated virus DNA. Proc Natl Acad Sci U S A. 1973 Jan;70(1):215–219. doi: 10.1073/pnas.70.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lai C. J., Dhar R., Khoury G. Mapping the spliced and unspliced late lytic SV40 RNAs. Cell. 1978 Aug;14(4):971–982. doi: 10.1016/0092-8674(78)90351-3. [DOI] [PubMed] [Google Scholar]
  13. Laughlin C. A., Myers M. W., Risin D. L., Carter B. J. Defective-interfering particles of the human parvovirus adeno-associated virus. Virology. 1979 Apr 15;94(1):162–174. doi: 10.1016/0042-6822(79)90446-x. [DOI] [PubMed] [Google Scholar]
  14. Leder P., Tilghman S. M., Tiemeier D. C., Polsky F. I., Seidman J. G., Edgell M. H., Enquist L. W., Leder A., Norman B. The cloning of mouse globin and surrounding gene sequences in bacteriophage lambda. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):915–920. doi: 10.1101/sqb.1978.042.01.093. [DOI] [PubMed] [Google Scholar]
  15. Meyer J., Neuwald P. D., Lai S. P., Maizel J. V., Jr, Westphal H. Electron microscopy of late adenovirus type 2 mRNA hybridized to double-stranded viral DNA. J Virol. 1977 Mar;21(3):1010–1018. doi: 10.1128/jvi.21.3.1010-1018.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nevins J. R., Darnell J. E., Jr Steps in the processing of Ad2 mRNA: poly(A)+ nuclear sequences are conserved and poly(A) addition precedes splicing. Cell. 1978 Dec;15(4):1477–1493. doi: 10.1016/0092-8674(78)90071-5. [DOI] [PubMed] [Google Scholar]
  17. Pinder J. C., Staynov D. Z., Gratzer W. B. Electrophoresis of RNA in formamide. Biochemistry. 1974 Dec 17;13(26):5373–5378. doi: 10.1021/bi00723a019. [DOI] [PubMed] [Google Scholar]
  18. Tal J., Ron D., Tattersall P., Bratosin S., Aloni Y. About 30% of minute virus of mice RNA is spliced out following polyadenylation. Nature. 1979 Jun 14;279(5714):649–651. doi: 10.1038/279649a0. [DOI] [PubMed] [Google Scholar]
  19. Tonegawa S., Maxam A. M., Tizard R., Bernard O., Gilbert W. Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1485–1489. doi: 10.1073/pnas.75.3.1485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Westphal H., Lai S. P. Quantitative electron microscopy of early adenovirus RNA. J Mol Biol. 1977 Nov 5;116(3):525–548. doi: 10.1016/0022-2836(77)90082-1. [DOI] [PubMed] [Google Scholar]
  21. Westphal H., Meyer J., Maizel J. V., Jr Mapping of adenovirus messenger RNA by electron microscopy. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2069–2071. doi: 10.1073/pnas.73.6.2069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. de la Maza L. M., Carter B. J. Adeno-associated virus DNA structure. Restriction endonuclease maps and arrangement of terminal sequences. Virology. 1977 Oct 15;82(2):409–430. doi: 10.1016/0042-6822(77)90016-2. [DOI] [PubMed] [Google Scholar]

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