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. 1979 Nov;76(11):5582–5586. doi: 10.1073/pnas.76.11.5582

Evidence for a dissociable protein subunit required for calmodulin stimulation of brain adenylate cyclase.

W A Toscano Jr, K R Westcott, D C LaPorte, D R Storm
PMCID: PMC411693  PMID: 293663

Abstract

An adenylate cyclase [ATP pyrophosphatelyase (cyclizing), EC 4.6.1.1] preparation that is not stimulated by NaF,5'-guanylyl imidodiphosphate, or Ca2+.calmodulin has been isolated from bovine cerebral cortex by Affi-Gel Blue chromatography and calmodulin-Sepharose chromatography. Sensitivity to these effectors was restored by incubation of the adenylate cyclase preparation with detergent-solubilized protein from bovine cerebral cortex. Reconstitution of of Ca2+.calmodulin activation required the presence of 5'-guanylyl imidodiphosphate. The factor required for restoration of Ca2+.calmodulin stimulation was sensitive to heat, trypsin digestion, and N-ethylmaleimide. These observations suggest that this adenylate cyclase activity requires the presence of one or more guanyl nucleotide binding subunits for calmodulin sensitivity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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