Abstract
Photobleaching in a two-dimensional periodic pattern has been used to characterize the motion of succinylconcanavalin A receptors on the surfaces of mouse fibroblasts. These receptors were found to exhibit anisotropic two-dimensional diffusion in adherent cells having parallel stress fibers. Diffusion coefficients of the order of 10(-11) cm2/sec and 10(-12) cm2/sec were measured for diffusive motions parallel and perpendicular to these fibers. The determination of the anisotropic motion used a two-dimensional Fourier analysis. A study was made of the effect of changes in cell shape on the photobleach pattern.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ash J. F., Louvard D., Singer S. J. Antibody-induced linkages of plasma membrane proteins to intracellular actomyosin-containing filaments in cultured fibroblasts. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5584–5588. doi: 10.1073/pnas.74.12.5584. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berlin R. D., Oliver J. M. Analogous ultrastructure and surface properties during capping and phagocytosis in leukocytes. J Cell Biol. 1978 Jun;77(3):789–804. doi: 10.1083/jcb.77.3.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bretscher M. S. Directed lipid flow in cell membranes. Nature. 1976 Mar 4;260(5546):21–23. doi: 10.1038/260021a0. [DOI] [PubMed] [Google Scholar]
- Dunham P. B., Goldstein I. M., Weissmann G. Potassium and amino acid transport in human leukocytes exposed to phagocytic stimuli. J Cell Biol. 1974 Oct;63(1):215–226. doi: 10.1083/jcb.63.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edelman G. M. Surface modulation in cell recognition and cell growth. Science. 1976 Apr 16;192(4236):218–226. doi: 10.1126/science.769162. [DOI] [PubMed] [Google Scholar]
- Gunther G. R., Wang J. L., Yahara I., Cunningham B. A., Edelman G. M. Concanavalin A derivatives with altered biological activities. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1012–1016. doi: 10.1073/pnas.70.4.1012. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nicolson G. L. Transmembrane control of the receptors on normal and tumor cells. I. Cytoplasmic influence over surface components. Biochim Biophys Acta. 1976 Apr 13;457(1):57–108. doi: 10.1016/0304-4157(76)90014-9. [DOI] [PubMed] [Google Scholar]
- Poo M. M., Poo W. J., Lam J. W. Lateral electrophoresis and diffusion of Concanavalin A receptors in the membrane of embryonic muscle cell. J Cell Biol. 1978 Feb;76(2):483–501. doi: 10.1083/jcb.76.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubenstein J. L., Smith B. A., McConnell H. M. Lateral diffusion in binary mixtures of cholesterol and phosphatidylcholines. Proc Natl Acad Sci U S A. 1979 Jan;76(1):15–18. doi: 10.1073/pnas.76.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith B. A., McConnell H. M. Determination of molecular motion in membranes using periodic pattern photobleaching. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2759–2763. doi: 10.1073/pnas.75.6.2759. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith L. M., Smith B. A., McConnell H. M. Lateral diffusion of M-13 coat protein in model membranes. Biochemistry. 1979 May 29;18(11):2256–2259. doi: 10.1021/bi00578a019. [DOI] [PubMed] [Google Scholar]
- Smolen J. E., Weissmann G. Mg2+-ATPase as a membrane ecto-enzyme of human granulocytes. Inhibitors, activators and response to phagocytosis. Biochim Biophys Acta. 1978 Oct 4;512(3):525–538. doi: 10.1016/0005-2736(78)90162-1. [DOI] [PubMed] [Google Scholar]
- Tsan M. F., Berlin R. D. Effect of phagocytosis on membrane transport of nonelectrolytes. J Exp Med. 1971 Oct 1;134(4):1016–1035. doi: 10.1084/jem.134.4.1016. [DOI] [PMC free article] [PubMed] [Google Scholar]