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. 1979 Nov;76(11):5660–5664. doi: 10.1073/pnas.76.11.5660

Globin synthesis and erythroid differentiation in a Friend cell variant deficient in heme synthesis.

T R Rutherford, P R Harrison
PMCID: PMC411709  PMID: 293668

Abstract

Friend erythroleukemia cells of line Fw are noninducible for hemoglobin synthesis by dimethyl sulfoxide, butyric acid, and other agents. However, these agents were found to induce hemoglobin synthesis if the cells were also treated with exogenous hemin. Butyric acid (or, to a lesser extent, dimethyl sulfoxide) by itself induced accumulation of the erythroid-specific membrane protein spectrin. The basis of the control of globin gene expression by hemin was investigated. Hemin does not control globin and total protein synthesis via the action of the hemin-controlled repressor. Rather, hemin, alone or in combination with dimethyl sulfoxide, induces accumulation of globin mRNA and nuclear globin RNA sequences. Direct measurements of heme synthesis indicate that Fw cells may be significantly deficient in heme metabolism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adamson S. D., Herbert E., Godchaux W. Factors affecting the rate of protein synthesis in lysate systems from reticulocytes. Arch Biochem Biophys. 1968 May;125(2):671–683. doi: 10.1016/0003-9861(68)90625-5. [DOI] [PubMed] [Google Scholar]
  2. Alter B. P., Goff S. C. Globin synthesis in mouse erythroleukemia cells in vitro: a switch in beta chains due to inducing agent. Blood. 1977 Nov;50(5):867–876. [PubMed] [Google Scholar]
  3. BRUNS G. P., LONDON I. M. THE EFFECT OF HEMIN ON THE SYNTHESIS OF GLOBIN. Biochem Biophys Res Commun. 1965 Jan 18;18:236–242. doi: 10.1016/0006-291x(65)90746-1. [DOI] [PubMed] [Google Scholar]
  4. Benoff S., Skoultchi A. I. X-linked control of hemoglobin production in somatic hybrids of mouse erythroleukemic cells and mouse lymphoma or bone marrow cells. Cell. 1977 Sep;12(1):263–274. doi: 10.1016/0092-8674(77)90204-5. [DOI] [PubMed] [Google Scholar]
  5. Beuzard Y., Rodvien R., London I. M. Effect of hemin on the synthesis of hemoglobin and other proteins in mammalian cells. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1022–1026. doi: 10.1073/pnas.70.4.1022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cimadevilla J. M., Hardesty B. Evidence for a non-hemin regulated translational repressor in Friend leukemia virus transformed murine proerythroblasts. Biochem Biophys Res Commun. 1975 Apr 21;63(4):931–937. doi: 10.1016/0006-291x(75)90658-0. [DOI] [PubMed] [Google Scholar]
  7. Clegg J. B., Naughton M. A., Weatherball D. J. Abnormal human haemoglobins. Separation and characterization of the alpha and beta chains by chromatography, and the determination of two new variants, hb Chesapeak and hb J (Bangkok). J Mol Biol. 1966 Aug;19(1):91–108. doi: 10.1016/s0022-2836(66)80052-9. [DOI] [PubMed] [Google Scholar]
  8. Cole R. J., Paul J. The effects of erythropoietin on haem synthesis in mouse yolk sac and cultured foetal liver cells. J Embryol Exp Morphol. 1966 Apr;15(2):245–260. [PubMed] [Google Scholar]
  9. Eisen H., Bach R., Emery R. Induction of spectrin in erythroleukemic cells transformed by Friend virus. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3898–3902. doi: 10.1073/pnas.74.9.3898. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Friend C., Preisler H. D., Scher W. Studies on the control of differentiation of murine virus-induced erythroleukemic cells. Curr Top Dev Biol. 1974;8:81–101. doi: 10.1016/s0070-2153(08)60606-7. [DOI] [PubMed] [Google Scholar]
  11. Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Glisin V., Crkvenjakov R., Byus C. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry. 1974 Jun 4;13(12):2633–2637. doi: 10.1021/bi00709a025. [DOI] [PubMed] [Google Scholar]
  13. Gusella J., Geller R., Clarke B., Weeks V., Housman D. Commitment to erythroid differentiation by friend erythroleukemia cells: a stochastic analysis. Cell. 1976 Oct;9(2):221–229. doi: 10.1016/0092-8674(76)90113-6. [DOI] [PubMed] [Google Scholar]
  14. Harrison P. R., Affara N., McNab A., Paul J. Erythroid differentiation in a Friend erythroleukemic cell X lymphoma hybrid cell line is limited, possibly due to reduced hem levels. Exp Cell Res. 1977 Oct 15;109(2):237–246. doi: 10.1016/0014-4827(77)90002-7. [DOI] [PubMed] [Google Scholar]
  15. Harrison P. R., Birnie G. D., Hell A., Humphries S., Young B. D., Paul J. Kinetic studies of gene frequency. I. Use of a DNA copy of reticulocyte 9 S RNA to estimate globin gene dosage in mouse tissues. J Mol Biol. 1974 Apr 25;84(4):539–554. doi: 10.1016/0022-2836(74)90115-6. [DOI] [PubMed] [Google Scholar]
  16. Harrison P. R., Rutherford T., Conkie D., Affara N., Sommerville J., Hissey P., Paul J. Analysis of erythroid differentiation in Friend cells using noninducible variants. Cell. 1978 May;14(1):61–70. doi: 10.1016/0092-8674(78)90301-x. [DOI] [PubMed] [Google Scholar]
  17. Hunter A. R. Control of haemoglobin synthesis. Effect of iron deprivation in reticulocytes on the distribution of ribosomes on globin mRNAs. Eur J Biochem. 1974 Sep 16;47(3):509–516. doi: 10.1111/j.1432-1033.1974.tb03719.x. [DOI] [PubMed] [Google Scholar]
  18. Legon S., Jackson R. J., Hunt T. Control of protein synthesis in reticulocyte lysates by haemin. Nat New Biol. 1973 Jan 31;241(109):150–152. doi: 10.1038/newbio241150a0. [DOI] [PubMed] [Google Scholar]
  19. Marchesi S. L., Steers E., Marchesi V. T., Tillack T. W. Physical and chemical properties of a protein isolated from red cell membranes. Biochemistry. 1970 Jan 6;9(1):50–57. doi: 10.1021/bi00803a007. [DOI] [PubMed] [Google Scholar]
  20. Mathews M. B., Hunt T., Brayley A. Specificity of the control of protein synthesis by haemin. Nat New Biol. 1973 Jun 20;243(129):230–233. doi: 10.1038/newbio243230a0. [DOI] [PubMed] [Google Scholar]
  21. Paul J., Hickey I. Haemoglobin synthesis in inducible, uninducible and hybrid Friend cell clones. Exp Cell Res. 1974 Jul;87(1):20–30. doi: 10.1016/0014-4827(74)90522-9. [DOI] [PubMed] [Google Scholar]
  22. Ross J., Sautner D. Induction of globin mRNA accumulation by hemin in cultured erythroleukemic cells. Cell. 1976 Aug;8(4):513–520. doi: 10.1016/0092-8674(76)90219-1. [DOI] [PubMed] [Google Scholar]
  23. Rovera G., Aden D., Surrey S. Allylisopropylacetamide restricts expression of beta minor globin gene in Friend cells. Nature. 1978 Mar 9;272(5649):172–175. doi: 10.1038/272172a0. [DOI] [PubMed] [Google Scholar]
  24. Tabuse Y., Furusawa M., Eisen H., Shibata K. Prostaglandin E1, an inducer of erythroid differentiation of Friend erythroleukemia cells. Exp Cell Res. 1977 Aug;108(1):41–45. [PubMed] [Google Scholar]
  25. Young B. D., Harrison P. R., Gilmour R. S., Birnie G. D., Hell A., Humphries S., Paul J. Kinetic studies of gene frequency. II. Complexity of globin complementary DNA and its hybridization characteristics. J Mol Biol. 1974 Apr 25;84(4):555–568. doi: 10.1016/0022-2836(74)90116-8. [DOI] [PubMed] [Google Scholar]
  26. Zucker W. V., Schulman H. M. Stimulation of globin-chain initiation by hemin in the reticulocyte cell-free system. Proc Natl Acad Sci U S A. 1968 Feb;59(2):582–589. doi: 10.1073/pnas.59.2.582. [DOI] [PMC free article] [PubMed] [Google Scholar]

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