Abstract
The distribution of prothoracicotropic hormone in the pupal brain of Manduca sexta has been determined by an in vitro assay for prothoracic gland activation. Prothoracicotropic activity was observed in both the brain and retrocerebral complex, but predominantly in the dorosolateral regions of the protocerebrum. Of the two groups of neurosecretory cells present in this area of the brain, only the two lateral type III neurosecretory cells exhibited significant prothoracicotropic hormone activity. Further analysis revealed that the neurohormone was localized in only one of the two type III cells, suggesting that a single neurosecretory cell in each hemisphere is the source of the hormone at the stage examined (day 0). Prothoracicotropic hormone activity was detected in both the corpora allata and the corpora cardiaca, but the corpora allata contained 2 to 9 times the activity of the corpora cardiaca, depending on developmental stage. The significantly higher level of activity in the corpora allata suggests that they may be the neurohemal organs through which the prothoracicotropic hormone of Manduca is released.
Keywords: neurohormone, prothoracic gland, corpora allata, corpora cardiaca, neurohemal organ
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- Bollenbacher W. E., Agui N., Granger N. A., Gilbert L. I. In vitro activation of insect prothoracic glands by the prothoracicotropic hormone. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5148–5152. doi: 10.1073/pnas.76.10.5148. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOMORI G. Aldehyde-fuchsin: a new stain for elastic tissue. Am J Clin Pathol. 1950 Jul;20(7):665–666. [PubMed] [Google Scholar]
- Gibbs D., Riddiford L. M. Prothoracicotropic hormone in Manduca sexta: localization by a larval assay. J Exp Biol. 1977 Feb;66(1):255–266. doi: 10.1242/jeb.66.1.255. [DOI] [PubMed] [Google Scholar]
- Goldsworthy G. J., Mordue W. Neurosecretory hormones in insects. J Endocrinol. 1974 Mar;60(3):529–558. doi: 10.1677/joe.0.0600529. [DOI] [PubMed] [Google Scholar]
- Ishizaki H. Changes in titer of the brain hormone during development of the silkworm. Bombyx mori. Dev Growth Differ. 1969 Jun;11(1):1–7. doi: 10.1111/j.1440-169x.1969.00001.x. [DOI] [PubMed] [Google Scholar]
- King D. S., Bollenbacher W. E., Borst D. W., Vedeckis W. V., O'connor J. D., Ittycheriah P. I., Gilbert L. I. The Secretion of alpha-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro. Proc Natl Acad Sci U S A. 1974 Mar;71(3):793–796. doi: 10.1073/pnas.71.3.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steel C. G. Some functions of identified neurosecretory cells in the brain of the aphid, Megoura viciae. Gen Comp Endocrinol. 1978 Feb;34(2):219–228. doi: 10.1016/0016-6480(78)90213-7. [DOI] [PubMed] [Google Scholar]
- Truman J. W., Riddiford L. M. Physiology of insect rhythms. 3. The temporal organization of the endocrine events underlying pupation of the tobacco hornworm. J Exp Biol. 1974 Apr;60(2):371–382. doi: 10.1242/jeb.60.2.371. [DOI] [PubMed] [Google Scholar]
- Weevers R. D. A lepidopteran saline: effects of inorganic cation concentrations on sensory, reflex and motor responses in a herbivorous insect. J Exp Biol. 1966 Feb;44(1):163–175. doi: 10.1242/jeb.44.1.163. [DOI] [PubMed] [Google Scholar]