Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Nov;76(11):5799–5803. doi: 10.1073/pnas.76.11.5799

Gene expression of an Escherichia coli ribosomal RNA promoter fused to structural genes of the galactose operon.

Y Ota, A Kikuchi, M Cashel
PMCID: PMC411738  PMID: 160557

Abstract

The promoter region of the rrnB ribosomal RNA gene of Escherichia coli has been ligated within the epimerase gene (galE) of the galactose operon in a lambda phage vector. The recombinant lambda phage has been characterized by restriction mapping and assays of both galK (galactokinase) gene activity and galactose messenger RNA hybridization. In such lyosgens, expression of the fused galactose operon occurs as a function of growth rate in a manner characteristic of ribosomal RNA gene expression and is subject to stringent control by amino acid availability for protein synthesis. Galactose messenger RNA arising from the ribosomal promoter is not as metabolically stable as ribosomal RNA.

Full text

PDF
5799

Images in this article

5802Image
on p.5802

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berman M. L., Beckwith J. Fusions of the lac operon to the transfer RNA gene tyrT of Escherichia coli. J Mol Biol. 1979 May 25;130(3):285–301. doi: 10.1016/0022-2836(79)90542-4. [DOI] [PubMed] [Google Scholar]
  2. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  3. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801–4805. doi: 10.1073/pnas.75.10.4801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dennis P. P., Nomura M. Regulation of the expression of ribosomal protein genes in Escherichia coli. J Mol Biol. 1975 Sep 5;97(1):61–76. doi: 10.1016/s0022-2836(75)80022-2. [DOI] [PubMed] [Google Scholar]
  5. Dennis P. P., Nomura M. Stringent control of the transcriptional activities of ribosomal protein genes in E. coli. Nature. 1975 Jun 5;255(5508):460–465. doi: 10.1038/255460a0. [DOI] [PubMed] [Google Scholar]
  6. Enquist L., Tiemeier D., Leder P., Weisberg R., Sternberg N. Safer derivatives of bacteriophage lambdagt-lambdaC for use in cloning of recombinant DNA molecules. Nature. 1976 Feb 19;259(5544):596–598. doi: 10.1038/259596a0. [DOI] [PubMed] [Google Scholar]
  7. Feiss M., Adyha S., Court D. L. Isolation of plaque-forming, galactose-transducing strains of phage lambda. Genetics. 1972 Jun;71(2):189–206. doi: 10.1093/genetics/71.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gilbert S. F., de Boer H. A., Nomura M. Identification of initiation sites for the in vitro transcription of rRNA operons rrnE and rrnA in E. coli. Cell. 1979 May;17(1):211–224. doi: 10.1016/0092-8674(79)90309-x. [DOI] [PubMed] [Google Scholar]
  9. Glaser G., Cashel M. In vitro transcripts from the rrn B ribosomal RNA cistron originate from two tandem promoters. Cell. 1979 Jan;16(1):111–121. doi: 10.1016/0092-8674(79)90192-2. [DOI] [PubMed] [Google Scholar]
  10. Glaser G., Enquist L., Cashel M. ColE1 cloning of a ribosomal RNA promoter region from lambdarifd18 by selection for lambda integration and excision functions. Gene. 1977;2(3-4):159–172. doi: 10.1016/0378-1119(77)90015-4. [DOI] [PubMed] [Google Scholar]
  11. Gottesman M. E., Yarmolinsky M. B. Integration-negative mutants of bacteriophage lambda. J Mol Biol. 1968 Feb 14;31(3):487–505. doi: 10.1016/0022-2836(68)90423-3. [DOI] [PubMed] [Google Scholar]
  12. Ketner G., Campbel A. Operator and promoter mutations affecting divergent transcription in the bio gene cluster of Escherichia coli. J Mol Biol. 1975 Jul 25;96(1):13–27. doi: 10.1016/0022-2836(75)90179-5. [DOI] [PubMed] [Google Scholar]
  13. Lazzarini R. A., Dahlberg A. E. The control of ribonucleic acid synthesis during amino acid deprivation in Escherichia coli. J Biol Chem. 1971 Jan 25;246(2):420–429. [PubMed] [Google Scholar]
  14. Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Neidhardt F. C. Roles of amino acid activating enzymes in cellular physiology. Bacteriol Rev. 1966 Dec;30(4):701–719. doi: 10.1128/br.30.4.701-719.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nomura M., Morgan E. A. Genetics of bacterial ribosomes. Annu Rev Genet. 1977;11:297–347. doi: 10.1146/annurev.ge.11.120177.001501. [DOI] [PubMed] [Google Scholar]
  17. OKAMOTO K., SUGINO Y., NOMURA M. Synthesis and turnover of phage messenger RNA in E. coli infected with bacteriophage T4 in the presence of chloromycetin. J Mol Biol. 1962 Nov;5:527–534. doi: 10.1016/s0022-2836(62)80126-0. [DOI] [PubMed] [Google Scholar]
  18. Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol. 1972 Feb 14;63(3):483–503. doi: 10.1016/0022-2836(72)90443-3. [DOI] [PubMed] [Google Scholar]
  19. Tiemeier D. C., Tilghman S. M., Leder P. Purification and cloning of a mouse ribosomal gene fragment in coliphage lambda. Gene. 1977;2(3-4):173–191. doi: 10.1016/0378-1119(77)90016-6. [DOI] [PubMed] [Google Scholar]
  20. Ullrich A., Shine J., Chirgwin J., Pictet R., Tischer E., Rutter W. J., Goodman H. M. Rat insulin genes: construction of plasmids containing the coding sequences. Science. 1977 Jun 17;196(4296):1313–1319. doi: 10.1126/science.325648. [DOI] [PubMed] [Google Scholar]
  21. Young R. A., Steitz J. A. Tandem promoters direct E. coli ribosomal RNA synthesis. Cell. 1979 May;17(1):225–234. doi: 10.1016/0092-8674(79)90310-6. [DOI] [PubMed] [Google Scholar]
  22. de Boer H. A., Gilbert S. F., Nomura M. DNA sequences of promoter regions for rRNA operons rrnE and rrnA in E. coli. Cell. 1979 May;17(1):201–209. doi: 10.1016/0092-8674(79)90308-8. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES