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. 1979 Nov;76(11):6009–6011. doi: 10.1073/pnas.76.11.6009

Intracellular dye-marked enkephalin neurons in the magnocellular preoptic nucleus of the goldfish hypothalamus.

T A Reaves Jr, J N Hayward
PMCID: PMC411783  PMID: 42909

Abstract

A method that combines intracellular recording, dye marking, and immunocytochemistry makes the study of functional and morphological aspects of enkephalin neurons in the magnocellular preoptic nucleus of the goldfish hypothalamus feasible. By use of multiple techniques, enkephalin neurons can be distinguished from other brain cells and can be reconstructed from drawings of serial sections containing the dye-injected opioid cells. These enkephalin cells and their processes measure 14-42 micron in somata diameter and are unipolar, bipolar, or multipolar. Their electrophysiological properties match those of other mammalian and fish magnocellular endocrine cells. This report confirms the one neuron-one hormone (peptide) hypothesis, supports synaptic over electronic coupling between enkephalin and adjacent hypothalamic neurons, and suggests that chemical and functional classification of single electrophysiologically and neuroanatomically studied central neurons can be achieved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bisset G. W., Chowdrey H. S., Feldberg W. Release of vasopressin by enkephalin. Br J Pharmacol. 1978 Mar;62(3):370–371. doi: 10.1111/j.1476-5381.1978.tb08472.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown K. T., Flaming D. G. Instrumentation and technique for beveling fine micropipette electrodes. Brain Res. 1975 Mar 14;86(1):172–180. doi: 10.1016/0006-8993(75)90652-6. [DOI] [PubMed] [Google Scholar]
  3. COONS A. H. Fluorescent antibody methods. Gen Cytochem Methods. 1958;1:399–422. [PubMed] [Google Scholar]
  4. Goossens N., Dierickx K., Vandesande F. Immunocytochemical localization of vasotocin and isotocin in the preopticohypophysial neurosecretory system of teleosts. Gen Comp Endocrinol. 1977 Aug;32(4):371–375. doi: 10.1016/0016-6480(77)90216-7. [DOI] [PubMed] [Google Scholar]
  5. Hayward J. N. Functional and morphological aspects of hypothalamic neurons. Physiol Rev. 1977 Jul;57(3):574–658. doi: 10.1152/physrev.1977.57.3.574. [DOI] [PubMed] [Google Scholar]
  6. Hayward J. N. Physiological and morphological identification of hypothalamic magnocellular neuroendocrine cells in goldfish preoptic nucleus. J Physiol. 1974 May;239(1):103–124. doi: 10.1113/jphysiol.1974.sp010558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hughes J., Smith T. W., Kosterlitz H. W., Fothergill L. A., Morgan B. A., Morris H. R. Identification of two related pentapeptides from the brain with potent opiate agonist activity. Nature. 1975 Dec 18;258(5536):577–580. doi: 10.1038/258577a0. [DOI] [PubMed] [Google Scholar]
  8. KANDEL E. R. ELECTRICAL PROPERTIES OF HYPOTHALAMIC NEUROENDOCRINE CELLS. J Gen Physiol. 1964 Mar;47:691–717. doi: 10.1085/jgp.47.4.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Koizumi K., Yamashita H. Studies of antidromically identified neurosecretory cells of the hypothalamus by intracellular and extracellular recordings. J Physiol. 1972 Mar;221(3):683–705. doi: 10.1113/jphysiol.1972.sp009776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reaves T. A., Jr, Hayward J. N. Immunocytochemical identification of enkephalinergic neurons in the hypothalamic magnocellular preoptic nucleus of the goldfish, Carassius auratus. Cell Tissue Res. 1979 Aug 3;200(1):147–151. doi: 10.1007/BF00236894. [DOI] [PubMed] [Google Scholar]
  11. Rossier J., Battenberg E., Pittman Q., Bayon A., Koda L., Miller R., Guillemin R., Bloom F. Hypothalamic enkephalin neurones may regulate the neurohypophysis. Nature. 1979 Feb 22;277(5698):653–655. doi: 10.1038/277653a0. [DOI] [PubMed] [Google Scholar]
  12. Sar M., Stumpf W. E., Miller R. J., Chang K. J., Cuatrecasas P. Immunohistochemical localization of enkephalin in rat brain and spinal cord. J Comp Neurol. 1978 Nov 1;182(1):17–37. doi: 10.1002/cne.901820103. [DOI] [PubMed] [Google Scholar]
  13. Simantov R., Snyder S. H. Opiate receptor binding in the pituitary gland. Brain Res. 1977 Mar 18;124(1):178–184. doi: 10.1016/0006-8993(77)90877-0. [DOI] [PubMed] [Google Scholar]
  14. Stewart W. W. Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer. Cell. 1978 Jul;14(3):741–759. doi: 10.1016/0092-8674(78)90256-8. [DOI] [PubMed] [Google Scholar]
  15. van Wimersma Greidanus T. B., Thody T. J., Verspaget H., de Rotte G. A., Goedemans H. J., Croiset G., van Ree J. M. Effects of morphine and beta-endorphin on basal and elevated plasma levels of alpha-MSH and vasopressin. Life Sci. 1979 Feb 12;24(7):579–585. doi: 10.1016/0024-3205(79)90153-x. [DOI] [PubMed] [Google Scholar]

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