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. 1979 Dec;76(12):6106–6109. doi: 10.1073/pnas.76.12.6106

Protein and phospholipid methylation during chemotaxis in Dictyostelium discoideum and its relationship to calcium movements.

J M Mato, D Marín-Cao
PMCID: PMC411811  PMID: 230497

Abstract

Suspensions of cyclic AMP sensitive cells of Dictyostelium discoideum responded to a cyclic AMP pulse with increased methylation of a protein of molecular weight about 120,000 and increased phospholipid demethylation. Protein methylation reached its peak 15-30 sec after cyclic AMP addition. Phospholipid demethylation reached its maximum within 2 min and basal levels were recovered in 3 min. S-Adenosyl-L-methionine is probably the methyl donor. In vitro addition of 0.25 mM and 25 microM S-adenosyl-L-methionine to sonicated D. discoideum cells inhibited ATP-dependent 45Ca2+ uptake by 70% and 25%, respectively. Based on these lines of evidence we propose that protein and phospholipid methylation are involved in D. discoideum chemotaxis probably by regulation of intracellular Ca2+ movements.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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