Abstract
The distribution of prostaglandin D synthetase activity was determined in various tissues of rat by using the supernatant fraction (10,000 x g, 20 min) of the homogenates. The highest activity was found in brain, spinal cord, and alimentary tract. The activity was uniquitously distributed in all parts of brain, and the highest specific activity was found in hypothalamus and thalamus. Homogenates of two neuroblastoma cell lines were found to produce prostaglandin D2, whereas a glioma cell line was almost inactive. Prostaglandin D2 is a potent and specific activator of the adenylate cyclase system of cultured neuroblastoma cells, suggesting the possibility that it may act as a neuromodulator in the central nervous system.
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Selected References
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- Abdel-Halim M. S., Anggård E. Regional and species differences in endogenous prostaglandin biosynthesis by brain homogenates. Prostaglandins. 1979 Mar;17(3):411–418. doi: 10.1016/s0090-6980(79)80009-x. [DOI] [PubMed] [Google Scholar]
- Abdel-Halim M. S., Hamberg M., Sjöquist B., Anggård E. Identification of prostaglandin D2 as a major prostaglandin in homogenates of rat brain. Prostaglandins. 1977 Oct;14(4):633–643. doi: 10.1016/0090-6980(77)90190-3. [DOI] [PubMed] [Google Scholar]
- Ali M., Cerskus A. L., Zamecnik J., McDonald J. W. Synthesis of prostaglandin D2 and thromboxane B2 by human platelets. Thromb Res. 1977 Oct;11(4):485–496. doi: 10.1016/0049-3848(77)90202-x. [DOI] [PubMed] [Google Scholar]
- Amano T., Hamprecht B., Kemper W. High activity of choline acetyltransferase induced in neuroblastoma x glia hybrid cells. Exp Cell Res. 1974 Apr;85(2):399–408. doi: 10.1016/0014-4827(74)90142-6. [DOI] [PubMed] [Google Scholar]
- Amano T., Richelson E., Nirenberg M. Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase-acetylcholinesterase-tyrosine hydroxylase-axons-dendrites). Proc Natl Acad Sci U S A. 1972 Jan;69(1):258–263. doi: 10.1073/pnas.69.1.258. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baker R. R., Thompson W. Positional distribution and turnover of fatty acids in phosphatidic acid, phosphinositides, phosphatidylcholine and phosphatidylethanolamine in rat brain in vivo. Biochim Biophys Acta. 1972 Aug 11;270(4):489–503. doi: 10.1016/0005-2760(72)90114-2. [DOI] [PubMed] [Google Scholar]
- Bloom F. E. Cyclic nucleotides in central synaptic function. Fed Proc. 1979 Jul;38(8):2203–2207. [PubMed] [Google Scholar]
- Christ-Hazelhof E., Nugteren D. H. Purification and characterisation of prostaglandin endoperoxide D-isomerase, a cytoplasmic, glutathione-requiring enzyme. Biochim Biophys Acta. 1979 Jan 29;572(1):43–51. doi: 10.1016/0005-2760(79)90198-x. [DOI] [PubMed] [Google Scholar]
- Christ-Hazelhof E., Nugteren D. H., Van Dorp D. A. Conversions of prostaglandin endoperoxides by glutathione-S-transferases and serum albumins. Biochim Biophys Acta. 1976 Dec 20;450(3):450–461. doi: 10.1016/0005-2760(76)90018-7. [DOI] [PubMed] [Google Scholar]
- Cicero T. J., Wilcox C. E., Meyer E. R. Effect of alpha-adrenergic blockers on naloxone-binding in brain. Biochem Pharmacol. 1974 Aug 15;23(16):2349–2352. doi: 10.1016/0006-2952(74)90570-x. [DOI] [PubMed] [Google Scholar]
- Collier H. O., Roy A. C. Morphine-like drugs inhibit the stimulation of E prostaglandins of cyclic AMP formation by rat brain homogenate. Nature. 1974 Mar 1;248(5443):24–27. doi: 10.1038/248024a0. [DOI] [PubMed] [Google Scholar]
- Ellis C. K., Smigel M. D., Oates J. A., Oelz O., Sweetman B. J. Metabolism of prostaglandin D2 in the monkey. J Biol Chem. 1979 May 25;254(10):4152–4163. [PubMed] [Google Scholar]
- Fitzpatrick F. A., Stringfellow D. A. Prostaglandin D2 formation by malignant melanoma cells correlates inversely with cellular metastatic potential. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1765–1769. doi: 10.1073/pnas.76.4.1765. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friesinger G. C., Oelz O., Sweetman B. J., Nies A. S., Data J. L. Prostaglandin D2, another renal prostaglandin? Prostaglandins. 1978 Jun;15(6):969–981. doi: 10.1016/0090-6980(78)90039-4. [DOI] [PubMed] [Google Scholar]
- Habig W. H., Pabst M. J., Jakoby W. B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974 Nov 25;249(22):7130–7139. [PubMed] [Google Scholar]
- Hamberg M., Fredholm B. B. Isomerization of prostaglandin H2 into prostaglandin D2 in the presence of serum albumin. Biochim Biophys Acta. 1976 Apr 22;431(1):189–183. doi: 10.1016/0005-2760(76)90273-3. [DOI] [PubMed] [Google Scholar]
- Honma M., Satoh T., Takezawa J., Ui M. An ultrasensitive method for the simultaneous determination of cyclic AMP and cyclic GMP in small-volume samples from blood and tissue. Biochem Med. 1977 Dec;18(3):257–273. doi: 10.1016/0006-2944(77)90060-6. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lunt G. G., Rowe C. E. The production of unesterified fatty acid in brain. Biochim Biophys Acta. 1968 Jul 1;152(4):681–693. doi: 10.1016/0005-2760(68)90114-8. [DOI] [PubMed] [Google Scholar]
- Nishizawa E. E., Miller W. L., Gorman R. R., Bundy G. L., Svensson J., Hamberg M. Prostaglandin d2 as a potential antithrombotic agent. Prostaglandins. 1975 Jan;9(1):109–121. doi: 10.1016/s0090-6980(75)80122-5. [DOI] [PubMed] [Google Scholar]
- Nugteren D. H., Hazelhof E. Isolation and properties of intermediates in prostaglandin biosynthesis. Biochim Biophys Acta. 1973 Dec 20;326(3):448–461. doi: 10.1016/0005-2760(73)90145-8. [DOI] [PubMed] [Google Scholar]
- Oelz O., Oelz R., Knapp H. R., Sweetman B. J., Oates J. A. Biosynthesis of prostaglandin D2. 1. Formation of prostaglandin D2 by human platelets. Prostaglandins. 1977 Feb;13(2):225–234. doi: 10.1016/0090-6980(77)90004-1. [DOI] [PubMed] [Google Scholar]
- Sabol S. L., Nirenberg M. Regulation of adenylate cyclase of neuroblastoma x glioma hybrid cells by alpha-adrenergic receptors. I. Inhibition of adenylate cyclase mediated by alpha receptors. J Biol Chem. 1979 Mar 25;254(6):1913–1920. [PubMed] [Google Scholar]
- Sharma S. K., Nirenberg M., Klee W. A. Morphine receptors as regulators of adenylate cyclase activity. Proc Natl Acad Sci U S A. 1975 Feb;72(2):590–594. doi: 10.1073/pnas.72.2.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shimizu T., Yamamoto S., Hayaishi O. Purification and properties of prostaglandin D synthetase from rat brain. J Biol Chem. 1979 Jun 25;254(12):5222–5228. [PubMed] [Google Scholar]