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. 1979 Dec;76(12):6250–6254. doi: 10.1073/pnas.76.12.6250

Cloning and expression of the Escherichia coli replication origin in a single-stranded DNA phage.

J Kaguni, L S LaVerne, D S Ray
PMCID: PMC411841  PMID: 392516

Abstract

The Escherichia coli DNA replication origin (oriC) and the adjacent asparagine synthetase gene (asnA) have been inserted into the duplex replicative form DNA of the single-stranded phage vector M13Goril. By in vitro recombination, the entire oriC asnA-containing plasmid pJS5 was inserted into M13Gori1 in both possible orientations. Both phage types transduce the asnA gene and confer upon the M13 vector the ability to replicate as a plasmid in the E. coli mutant rep3. In rep+ hosts, these phages undergo single-stranded DNA synthesis and viral morphogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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