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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Dec;76(12):6260–6264. doi: 10.1073/pnas.76.12.6260

Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase.

R L Erikson, M S Collett, E Erikson, A F Purchio
PMCID: PMC411843  PMID: 230504

Abstract

The avian sarcoma virus transforming gene product pp60src has been partially purified by using ion exchange or immunoaffinity chromatography. These preparations contain a cyclic AMP-independent protein kinase activity capable of transferring radiolabel from [gamma-32P]ATP to immune rabbit IgG, casein, and the heavy chain purified from normal rabbit IgG. The casein kinase activity is specifically inhibited by anti-pp60src IgG. Comparison of the pp60src-protein kinase isolated from cells transformed by a wild-type ASV or by an ASV temperature-sensitive transformation mutant revealed that the latter product had a half-life of 3 min at 41 degrees C whereas that of the wild-type product was 20 min.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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