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. 1979 Dec;76(12):6279–6283. doi: 10.1073/pnas.76.12.6279

Identification of chorion protein precursors and the mRNAs that encode them in Drosophila melanogaster.

G Thireos, R G Shea, F C Kafatos
PMCID: PMC411847  PMID: 118465

Abstract

Specific antisera were generated for major size classes of Drosophila chorion proteins. These antisera were used in conjunction with cell-free translation of follicular RNA to identify precursors, containing signal peptides, for chorion components A1, A2, B1, and B2. An additional precursor cross-reacts with anti-B antisera, although its product appears to correspond to a protein distinct from authenic B proteins. The size classes of mRNA encoding A and B protein precursors have been identified by cell-free translations.

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Selected References

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  1. Beadle G. W., Ephrussi B. The Differentiation of Eye Pigments in Drosophila as Studied by Transplantation. Genetics. 1936 May;21(3):225–247. doi: 10.1093/genetics/21.3.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Kessler S. W. Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent. J Immunol. 1976 Nov;117(5 Pt 1):1482–1490. [PubMed] [Google Scholar]
  4. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  5. Petri W. H., Wyman A. R., Kafatos F. C. Specific protein synthesis in cellular differentiation. III. The eggshell proteins of Drosophila melanogaster and their program of synthesis. Dev Biol. 1976 Mar;49(1):185–199. doi: 10.1016/0012-1606(76)90266-9. [DOI] [PubMed] [Google Scholar]
  6. Regier J. C., Kafatos F. C., Goodfliesh R., Hood L. Silkmoth chorion proteins: sequence analysis of the products of a multigene family. Proc Natl Acad Sci U S A. 1978 Jan;75(1):390–394. doi: 10.1073/pnas.75.1.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Robb J. A. Maintenance of imaginal discs of Drosophila melanogaster in chemically defined media. J Cell Biol. 1969 Jun;41(3):876–885. doi: 10.1083/jcb.41.3.876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Shields D., Blobel G. Efficient cleavage and segregation of nascent presecretory proteins in a reticulocyte lysate supplemented with microsomal membranes. J Biol Chem. 1978 Jun 10;253(11):3753–3756. [PubMed] [Google Scholar]
  9. Spradling A. C., Mahowald A. P. Identification and genetic localization of mRNAs from ovarian follicle cells of Drosophila melanogaster. Cell. 1979 Mar;16(3):589–598. doi: 10.1016/0092-8674(79)90032-1. [DOI] [PubMed] [Google Scholar]
  10. Spradling A. C., Waring G. L., Mahowald A. P. Drosophila bearing the ocelliless mutation underproduce two major chorion proteins both of which map near this gene. Cell. 1979 Mar;16(3):609–616. doi: 10.1016/0092-8674(79)90034-5. [DOI] [PubMed] [Google Scholar]
  11. Spradling A., Pardue M. L., Penman S. Messenger RNA in heat-shocked Drosophila cells. J Mol Biol. 1977 Feb 5;109(4):559–587. doi: 10.1016/s0022-2836(77)80091-0. [DOI] [PubMed] [Google Scholar]
  12. Vaitukaitis J., Robbins J. B., Nieschlag E., Ross G. T. A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol Metab. 1971 Dec;33(6):988–991. doi: 10.1210/jcem-33-6-988. [DOI] [PubMed] [Google Scholar]
  13. Waring G. L., Mahowald A. P. Identification and time of synthesis of chorion proteins in Drosophila melanogaster. Cell. 1979 Mar;16(3):599–607. doi: 10.1016/0092-8674(79)90033-3. [DOI] [PubMed] [Google Scholar]

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