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IFN-γ-production after immunization. Samples were taken 2 weeks after each immunization. Peripheral blood mononuclear cells (PBMCs) were isolated by a standard percoll separation technique and assessed for H5HA antigen- (A) M2eNP antigen- (B) and NA antigen- (C) specific responses by ELISpot. (D) Average SFCs, spot-forming cell levels of IFN-γ-producing cells after third stimulation with H5HA, M2eNP, or NA antigen.
