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. 1979 Dec;76(12):6435–6438. doi: 10.1073/pnas.76.12.6435

Cytoskeleton-disrupting drugs enhance effect of growth factors and hormones on initiation of DNA synthesis.

A M Otto, A Zumbé, L Gibson, A M Kubler, L Jimenez de Asua
PMCID: PMC411879  PMID: 293731

Abstract

Addition of growth factors, such as prostaglandin F2 alpha or fibroblastic growth factor, to quiescent Swiss mouse 3T3 cells resulted in an abrupt increase in the rate of initiation of DNA synthesis after a lag phase of 13-15 hr. This increase could be quantified by a rate constant k. Addition of colchicine, Colcemid, or vinblastine had a synergistic effect on the initiation of DNA synthesis triggered by PGF2 alpha or FGF by increasing the value of k. These drugs alone had no effect. Colchicine had a synergistic effect only if added within 8 hr of the PGF2 alpha or FGF addition. Also, colchicine exerted its full effect when it was present only for the first 5 hr with either growth factor. These results suggest that an intact cytoskeleton is not required for the initiation of DNA synthesis. Furthermore, cytoskeleton-disrupting drugs enhance the stimulatory effect of the growth factors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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