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. 1979 Dec;76(12):6582–6586. doi: 10.1073/pnas.76.12.6582

Differential effects of colchicine and cytochalasins on the shedding of murine B cell membrane IgM and IgD.

S G Emerson, R E Cone
PMCID: PMC411910  PMID: 316543

Abstract

Lactoperoxidase (EC 1.11.1.7) catalyzed cell surface radioiodination was employed to monitor the fate of murine B cell membrane (mem) IgM and IgD on radiolabeled cells in short-term culture. Both mem-IgM and mem-IgD were shed from the cell surface with biphasic kinetics. The rapid phase of mem-IgD shedding was somewhat slower (half-time = 12 hr) than that of mem-IgM shedding (half-time = 7-8 hr). The effect of temperature, colchicine, and cytochalasin on the shedding of the two membrane immunoglobulin isotypes was determined. The shedding of mem-IgD was more energy dependent than that of mem-IgM and was sensitive to colchicine but not cytochalasin. Conversely, the shedding of mem-IgM was sensitive to cytochalasin but not colchicine. The results suggest that the mechanisms of shedding of mem-IgM and mem-IgD are qualitatively distinct and may be regulated by microfilaments or microtubules, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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