Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Dec;76(12):6646–6648. doi: 10.1073/pnas.76.12.6646

Immunofluorescent localization of angiotensin converting enzyme in epithelioid and giant cells of sarcoidosis granulomas.

E Silverstein, L P Pertschuk, J Friedland
PMCID: PMC411924  PMID: 230518

Abstract

Angiotensin converting enzyme (ACE) (dipeptidyl carboxypeptidase; peptidyldipeptide hydrolase, EC 3.4.15.1) was localized in epithelioid and giant cells within ACE-rich sarcoidosis granulomas, but not control granulomas, by immunofluorescence by using a rabbit anti-human ACE immunoglobulin for localization and fluorescein-labeled goat anti-rabbit immunoglobulin for detection. ACE was frequently not detected in the very central epithelioid cells, perhaps due to a paucity of cellular or molecular stimulatory interaction in this region. The results suggest that marked elevation of ACE in the granulomas and often in the serum in sarcoidosis may be due to intense induction of ACE in most epithelioid and giant cells of the sarcoidosis granulomas.

Full text

PDF
6646

Images in this article

6647Image
on p.6647
6647Image
on p.6647

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caldwell P. R., Seegal B. C., Hsu K. C., Das M., Soffer R. L. Angiotensin-converting enzyme: vascular endothelial localization. Science. 1976 Mar 12;191(4231):1050–1051. doi: 10.1126/science.175444. [DOI] [PubMed] [Google Scholar]
  2. Carr I., Norris P. The fine structure of human macrophage granules in sarcoidosis. J Pathol. 1977 May;122(1):29–32. doi: 10.1002/path.1711220106. [DOI] [PubMed] [Google Scholar]
  3. Friedland J., Setton C., Silverstein E. Angiotensin converting enzyme: induction by steroids in rabbit alveolar macrophages in culture. Science. 1977 Jul 1;197(4298):64–65. doi: 10.1126/science.194311. [DOI] [PubMed] [Google Scholar]
  4. Friedland J., Setton C., Silverstein E. Induction of angiotensin converting enzyme in human monocytes in culture. Biochem Biophys Res Commun. 1978 Aug 14;83(3):843–849. doi: 10.1016/0006-291x(78)91471-7. [DOI] [PubMed] [Google Scholar]
  5. Friedland J., Silverstein E. A sensitive fluorimetric assay for serum angiotensin-converting enzyme. Am J Clin Pathol. 1976 Aug;66(2):416–424. doi: 10.1093/ajcp/66.2.416. [DOI] [PubMed] [Google Scholar]
  6. Hinman L. M., Stevens C., Matthay R. A., Bernard J., Gee L. Angiotensin convertase activities in human alveolar macrophages: effects of cigarette smoking and sarcoidosis. Science. 1979 Jul 13;205(4402):202–203. doi: 10.1126/science.221980. [DOI] [PubMed] [Google Scholar]
  7. Lieberman J., Beutler E. Elevation of serum angiotensin-converting enzyme in Gaucher's disease. N Engl J Med. 1976 Jun 24;294(26):1442–1444. doi: 10.1056/NEJM197606242942609. [DOI] [PubMed] [Google Scholar]
  8. Lieberman J. Elevation of serum angiotensin-converting-enzyme (ACE) level in sarcoidosis. Am J Med. 1975 Sep;59(3):365–372. doi: 10.1016/0002-9343(75)90395-2. [DOI] [PubMed] [Google Scholar]
  9. Ryan U. S., Ryan J. W., Whitaker C., Chiu A. Localization of angiotensin converting enzyme (kininase II). II. Immunocytochemistry and immunofluorescence. Tissue Cell. 1976;8(1):125–145. doi: 10.1016/0040-8166(76)90025-2. [DOI] [PubMed] [Google Scholar]
  10. Schmitt E., Meuret G., Stix L. Monocyte recruitment in tuberculosis and sarcoidosis. Br J Haematol. 1977 Jan;35(1):11–17. doi: 10.1111/j.1365-2141.1977.tb00557.x. [DOI] [PubMed] [Google Scholar]
  11. Silverstein E., Friedland J. Elevated serum and spleen angiotensin converting enzyme and serum lysozyme in Gaucher's disease. Clin Chim Acta. 1977 Jan 3;74(1):21–25. doi: 10.1016/0009-8981(77)90382-5. [DOI] [PubMed] [Google Scholar]
  12. Silverstein E., Friedland J., Kitt M., Lyons H. A. Increased serum angiotensin converting enzyme activity in sarcoidosis. Isr J Med Sci. 1977 Oct;13(10):995–1000. [PubMed] [Google Scholar]
  13. Silverstein E., Friedland J., Lyons H. A., Gourin A. Elevation of angiotensin-converting enzyme in granulomatous lymph nodes and serum in sarcoidosis: clinical and possible pathogenic significance. Ann N Y Acad Sci. 1976;278:498–513. doi: 10.1111/j.1749-6632.1976.tb47062.x. [DOI] [PubMed] [Google Scholar]
  14. Silverstein E., Friedland J., Lyons H. A., Gourin A. Markedly elevated angiotensin converting enzyme in lymph nodes containing non-necrotizing granulomas in sarcoidosis. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2137–2141. doi: 10.1073/pnas.73.6.2137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Silverstein E., Friedland J., Lyons H. A. Serum angiotensin converting enzyme in sarcoidosis: clinical significance. Isr J Med Sci. 1977 Oct;13(10):1001–1006. [PubMed] [Google Scholar]
  16. Silverstein E., Friedland J., Setton C. Angiotensin-converting enzyme in macrophages and Freund's adjuvant granuloma. Isr J Med Sci. 1978 Mar;14(3):314–318. [PubMed] [Google Scholar]
  17. Silverstein E., Friedland J., Vuletin J. C. Marked elevation of serum angiotension-converting enzyme and hepatic fibrosis containing long-spacing collagen fibrils in type 2 acute neuronopathic Gaucher's disease. Am J Clin Pathol. 1978 Apr;69(4):467–471. doi: 10.1093/ajcp/69.4.467. [DOI] [PubMed] [Google Scholar]
  18. Silverstein E. Pathogenesis of sarcoidosis: an hypothetical model. Med Hypotheses. 1976 May-Jun;2(3):75–78. doi: 10.1016/0306-9877(76)90047-5. [DOI] [PubMed] [Google Scholar]
  19. Soffer R. L. Angiotensin-converting enzyme and the regulation of vasoactive peptides. Annu Rev Biochem. 1976;45:73–94. doi: 10.1146/annurev.bi.45.070176.000445. [DOI] [PubMed] [Google Scholar]
  20. Soler P., Basset F. Morphology and distribution of the cells of a sarcoid granuloma: ultrastructural study of serial sections. Ann N Y Acad Sci. 1976;278:147–160. doi: 10.1111/j.1749-6632.1976.tb47026.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES