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. 1979 Dec;76(12):6652–6655. doi: 10.1073/pnas.76.12.6652

Polyamine-pyridoxal Schiff bases in urine.

R Aigner-Held, R A Campbell, G D Daves Jr
PMCID: PMC411926  PMID: 293750

Abstract

Schiff bases of the diamines 1,3-diaminopropane, putrescine, and cadaverine and the polyamines spermidine and spermine with pyridoxal or pyridoxal phosphate occur in human urine, as shown by gas chromatographic/mass spectrometric selected ion-monitoring techniques. By use of synthetic standards, procedures were devised for conversion of the Schiff bases to stable derivatives amenable to gas chromatographic/mass spectrometric analysis. These procedures involve borohydride reduction of the C = N double bond, hydrolytic removal of the phosphate group, chromatographic separation from the bulk of urinary constituents, and trifluoroacetylation of polar functional groups. The levels of the polyamine-pyridoxal Schiff bases were estimated to be in the range of pmol/ml or urine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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