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. Author manuscript; available in PMC: 2015 Sep 26.
Published in final edited form as: Oncogene. 2014 Apr 28;34(13):1718–1728. doi: 10.1038/onc.2014.93

Figure 1. ORCTL3 induces apoptosis in transformed but not in normal CV-1 kidney cells.

Figure 1

(a-f) ORCTL3, ORCTL3ER, Luc, and Caspase 2 were transfected into the H-ras-, myc-, E6-, and P53-transformed CV-1 cells as well as into E1A- and empty vector-transfected CV-1 cells. Apoptosis was monitored after 24 hours. Data represent the means of independent experiments ± SD, (n=3). (g) ORCTL3 is more efficiently expressed in WT CV-1 compared to transformed CV-1 cells. WT and transformed CV-1 cells transfected with HA-tagged ORCTL3 were harvested 24 hours after transfection and stained with an Alexa-488 conjugated antibody against HA. Data represent the means of independent experiments ± SD, (n=3). (h) Cells transfected with a plasmid expressing GFP were harvested 24 hours after transfection and assayed by flow cytometry to quantify the GFP signals. Each bar represents the mean ± SD, (n=3).