Abstract
Almost all invasive Neisseria meningitidis isolates express capsular polysaccharide. Antibody (Ab) is required for complement-dependent killing of meningococci. While alternative pathway evasion has received considerable attention, little is known about classical pathway (CP) inhibition by meningococci and forms the basis of this study. We engineered capsulated and unencapsulated isogenic mutant strains of groups A, B, C, W and Y meningococci to express similar amounts of the same factor H-binding protein (fHbp; a key component of group B meningococcal vaccines) molecule. Despite similar anti-fHbp mAb binding, significantly less C4b was deposited on all five encapsulated mutants compared to their unencapsulated counterparts (P<0.01), when purified C1 and C4 were used to deposit C4b. Reduced C4b deposition was the result of capsule-mediated inhibition of C1q engagement by Ab. C4b deposition correlated linearly with C1q engagement by anti-fHbp. While B, C, W and Y capsules limited CP-mediated killing by anti-fHbp, the unencapsulated group A mutant paradoxically was more resistant than its encapsulated counterpart. Strains varied considerably in their susceptibility to anti-fHbp and complement despite similar Ab binding, which may have implications for the activity of fHbp-based vaccines. Capsule also limited C4b deposition by anti-porin A mAbs. Capsule expression decreased binding of an anti-LOS IgM mAb (~1.2 to 2-fold reduction in fluorescence). Akin to observations with IgG, capsule also decreased IgM-mediated C4b deposition when IgM binding to the mutant strain pairs was normalized. In conclusion, we show that capsular polysaccharide, a critical meningococcal virulence factor, inhibits the CP of complement.
Keywords: Neisseria meningitidis, meningococci, capsular polysaccharide, complement, classical pathway, antibody, C4, C1q
Introduction
Complement-dependent bactericidal activity is an established correlate of protection against meningococcal disease. Intrathecal serotherapy with equine anti-meningococcal antiserum was shown to be beneficial in meningococcal meningitis as far back as the early 1900s by Flexner and Jobling (1, 2), which suggested a role for antibody (Ab) in host defenses against invasive disease. Elegant studies by Goldschneider and colleagues firmly established a role for Ab in protection against invasive meningococcal infection (3). They reported an inverse relationship between the incidence of meningococcal disease and age-specific serum bactericidal activity against prototypic groups A, B and C meningococcal strains (3). These findings were corroborated by a more recent study in the U.K (4).
While colonization of the human nasopharynx by Neisseria meningitidis is a relatively common event, invasive disease is rare (5). Previously ‘nonimmune’ individuals may be protected against invasive infection if colonization with Neisseria meningitidis elicits adequate titers of protective Ab (6). With a few exceptions (7–9), almost every strain of N. meningitidis recovered from the bloodstream or cerebrospinal fluid expresses a polysaccharide capsule. By contrast, unencapsulated (“capsule null”, or cnl) isolates comprise ~15–20 % of meningococci isolated from the nasopharynges of asymptomatic carriers (10, 11). Capsule appears to be critical for invasion of the bacteria into the bloodstream of individuals who lack adequate titers of specific protective Ab, where high levels of complement are encountered.
Over the past several years, much attention has focused on the role of membrane proteins in complement evasion, in particular the interactions between factor H (FH), an inhibitor of the alternative pathway of complement, and meningococcal factor H binding protein (fHbp (12); also referred to as LP2086 (13)) (14–16). fHbp is a component of a group B meningococcal vaccine candidate that has recently been licensed for clinical use in several countries (17). Other meningococcal surface molecules that inhibit the alternative pathway include Neisserial surface protein A (NspA) (18) and PorB2 (19), which directly bind to FH. Lipooligosaccharide (LOS) sialic acid, in concert with surface-bound C3 fragments increases FH recruitment (20). Capsular polysaccharide is critical for meningococci to evade killing by high levels of complement and loss of capsule expression (alone) is associated with a dramatic decrease in complement resistance (21, 22) and virulence in the infant rat and infant mouse models (23, 24).
The role of sialic acids, such as N-acetyl neuraminic acid, in regulating the alternative pathway of complement on host cells (25, 26) and on microbial surfaces (27, 28) including N. meningitidis (20, 29–32), is well described. Capsules expressed by four of the five most widely distributed meningococcal groups (groups B, C, W and Y) contain N-acetyl neuraminic acid and in light of the well-established link between sialic acids and the alternative pathway, studies pertaining to complement regulation by capsules have also focused on the alternative pathway. Given the importance of the classical pathway (CP) for complement-dependent killing of meningococci (22, 33) and the central role of capsule in serum resistance, we sought to determine how capsule modulated early events in Ab-dependent CP activation.
Materials and Methods
Bacterial strains and mutants
Isogenic mutant strains were derived from N. meningitidis strains A2594 (WUE2594), H44/76, C2120, W171 and Y2225. Their relevant characteristics are listed in Table 1. LOS sialylation was abrogated by deleting lst (encodes lipooligosaccharide sialyltransferase) as described previously (18). Note that group A isolates do not sialylate their LOS unless supplied with cytidinemonophospho-N-acetyl neuraminic acid (CMP-Neu5Ac) in growth media. To delete capsule from group A strain 2594, mynB (its newly proposed designation is csaB (34)) was deleted as described previously (18). The groups B, C W and Y isolates were rendered unencapsulated by deleting their respective polysialyltransferase siaD genes (19, 33) – these genes are now designated csb, csc, csw and csy, respectively (34). fHbp from A2594, C2120, W171 and Y2220 was replaced with fHbp from H44/76 (variant 1 fHbp) as follows. We previously constructed a mutant of group Y strain Y2220, called Y2220 siaDH44/76GNA1870+ (fHbp was previously called Genome-derived Neisseria Antigen (GNA) 1870 (12)) that expresses the full length H44/76 fHbp (14). This mutant contains the tetracycline-resistance marker immediately 3′ to fHbp. DNA extracted from Y2220 siaDH44/76GNA1870+ was used to transform encapsulated strains that lacked LOS sialylation (A2594, C2120 lst, W171 lst and Y2225 lst) and unencapsulated / LOS unsialylated mutants A2594 mynB, C2120 siaD lst, W171 siaD lst and Y2225 siaD lst. Tetracycline-resistant colonies were selected (the MIC of tetracycline for each strain was determined) and screened for binding to mAb JAR 1 that is specific for variant 1 fHbp expressed by H44/76 (35). The fHbp of clones that bound to mAb JAR 1 was sequenced to verify the presence of the entire H44/76 fHbp sequence. For simplicity, we will henceforth refer to the strains by their original serogroup (i.e., A, B, C, W or Y) followed by Cap+ or Cap− to denote encapsulated and unencapsulated mutants, respectively. All mutants used in this study lack LOS sialic acid and express the same variant 1 fHbp molecule.
Table 1.
Strain | Group | Serotype (Class) |
SerosubtypeA | MLST | Origin (Year) |
Ref. |
---|---|---|---|---|---|---|
A2594 (WUE 2594) | A | 4 (PorB3) | 1–9 | ST-5 | Germany, 1991 | (63) |
H44/76 | B | 15 (PorB3) | 1.7,16 | ST-32 | Norway, 1976 | (64) |
C2120 | C | NTB (PorB2) | 1.5,2 | ST-11 | NAC | (65) |
W171 | W | NT (PorB2) | 1.10 | ST-11/CC-11 | USA | (66) |
Y2225 | Y | NTB (PorB2) | 1.2 | ST-11/CC-11 | Germany 1994 | (66) |
defined either by anti-PorA mAb reactivity or by variable region (VR) sequence typing
NT, Non-typeable
NA, not available
Complement proteins
Purified C1 complex (200 µg/ml), C1q (1 mg/ml) and C4 (4 mg/ml) were from Complement Technology, Inc (Tyler, TX). Protease inhibitors in purified C1 (EDTA, benzamidine and EACA) were removed by dialysis at 4 °C against PBS using an Amicon® Ultra-4 centrifugal Filter Unit (30 kD cutoff) prior to use.
IgG and IgM depleted serum
IgG and IgM were depleted from serum as described previously by passage over protein G sepharose and anti-human IgM agarose (36). Briefly, serum that was treated with EDTA (10 mM) to prevent complement activation and 1M NaCl to minimize C1q loss during immunodepletion was passed over immobilized protein G and anti-human IgM at 4 °C. The estimated amount of IgG and IgM in serum did not exceed the binding capacity of the columns. The fall-through was spin-concentrated and dialyzed against PBS with an Amicon® Ultra-15 (30 kD cutoff). Hemolytic activity was verified with the Total Hemolytic Complement kit (The Binding Site, Birmingham, U.K). IgG and IgM depletion was ascertained by dot blot assays on PVDF membranes as described previously (36). Serum was stored in single-use aliquots at −70 °C.
Antibodies and conjugates
Anti-variant 1 fHbp mAb JAR 1 (mouse IgG3) has been described previously (35). For simplicity, JAR 1 will henceforth be called anti-fHbp in this paper. Anti-PorA mAbs 1.9, 1.7, 1.5 and 1.2 that recognize PorA expressed by A2594, H44/76, C2120 and Y2225, respectively, were obtained from the National Institute for Biological Standards and Control (NIBSC), Hertfordshire, U.K. mAb P1.9 was supplied as ascites, while the other anti-PorA mAbs were provided as concentrated tissue culture supernatants. The mAb 3F11 hybridoma cell line was provided by Dr. Michael A. Apicella (University of Iowa); mAb 3F11 (mouse IgM) recognizes the lacto-N-neotetraose LOS species in the unsialylated state (37, 38). Polyclonal chicken anti-human C4 biotin used in flow cytometry assays was from Cedarlane Laboratories. Sheep anti-human C1q-FITC was from Biodesign (MP Biomedicals). Sheep polyclonal anti-human C4 used in western blotting assays was from Biodesign (MP Biomedicals). FITC-conjugated anti-mouse IgG, IgM and extravidin, and alkaline phosphatase conjugated anti-sheep IgG were from Sigma. All conjugates for flow cytometry were used at a dilution of 1:200 in PBS/1% BSA and anti-sheep IgG ALP was used at 1:1000 in PBS/0.05% Tween 20. Function blocking anti-factor Bb mAb (Quidel Corporation; Cat. No. A227) was added to serum at a concentration of 100 µg/ml to block alternative pathway activation (39, 40).
Flow cytometry
Flow cytometry to detect bound Ab, C4b or C1q was performed using previously described methods (18–20). To detect C4b deposition using pure complement components, ~107 bacteria in HBSS containing 1 mM Ca2+ and 1mM Mg2+ (HBSS++) were incubated with anti-fHbp (10 µg/ml) or the indicated dilutions of anti-PorA or mAb 3F11, followed by C4 (50 µg/ml) and C1 complex (7.5 µg/ml) in a final reaction volume of 50 µl. Control reactions that lacked anti-fHbp were included to compare C4b deposition that was mediated by Ab versus C4b deposition that may have occurred independently of Ab binding – for example, by direct binding of C1 to bacteria. Following incubation of the reaction mixture for 30 min at 37 °C, bacteria were washed and deposited C4b was measured using anti-C4 biotin and extravidin FITC. To measure C1q engagement by anti-fHbp, bacteria were incubated with anti-fHbp (10 µg/ml) and purified C1q (20 µg/ml) for 30 min at 37 °C, washed and bound C1q was detected with anti-C1q FITC. Data were collected on a BD FACSCalibur (Becton Dickinson) flow cytometer and analyzed with FlowJo software (Tree Star).
Western blotting
C4b deposited on bacteria was analyzed by western blotting as described previously (41, 42). Bacteria were incubated with anti-fHbp, C1 and C4 as described above, washed thrice and pellets were lysed with 4× LDS sample buffer containing 2-ME. The lysates were electrophoresed on 4–12% Bis-Tris gels and transferred to a PVDF membrane by western blotting. Blots were cut horizontally at the 50 kD marker; proteins that migrated above this were probed with sheep anti-human C4 to detect adducts of the C4b α' chain covalently linked to its bacterial targets, while proteins migrating faster than the 50kD marker were stained with coomassie blue, which served as a control to indicate equal loading of Cap+ and Cap− bacterial lysates.
Serum bactericidal assay
Serum bactericidal assays were performed as described previously (40). Bacteria that had been harvested from an overnight culture on chocolate agar plates were repassaged onto fresh chocolate agar and allowed to grow for 4–5 h at 37°C in an atmosphere containing 5% CO2 and then suspended in HBSS++. Approximately 2000 CFU of N. meningitidis with anti-fHbp (0.25 µg/ml) were incubated with IgG/IgM-depleted serum (5%) that contained 100 µg/ml of the anti-factor Bb mAb, in a final reaction volume of 75 µl. This concentration of mAb and complement was determined following preliminary titration experiments. Aliquots of 12.5-µl reaction mixtures were plated onto chocolate agar in duplicate at the beginning of the assay (t0) and again after incubation at 37°C for 30 min (t30). Survival was calculated as the number of viable colonies at t30 relative to t0.
Results
Capsule inhibits C4b deposition by anti-fHbp mAb JAR 1
To determine the effect of capsule on CP activation mediated by an anti-fHbp mAb, we replaced the fHbp molecule of the isogenic pairs of strains – A2594 and A2594 mynB, C2120 lst and C2120 siaD lst, W171 lst and W171 siaD lst and Y2225 lst and Y2225 siaD lst – with the variant 1 fHbp molecule of strain H44/76. We elected to use mutants lacking LOS sialic acid (lst mutants) because LOS is a target for C4b deposition on the pathogenic Neisseriae (41, 42) and LOS sialylation also limits C4b deposition on bacteria (42). Further, deleting capsule (siaD mutants) on groups B, C, W and Y isolates may ‘shunt’ cytidinemonophospho-N-acetyl neuraminic acid (CMP-NANA) from capsule biosynthesis on to LOS and result in a greater proportion of sialylated LOS on Cap− strains (22). As shown in Figure 1, all mutants bound similar amounts of anti-fHbp by flow cytometry.
Bacteria were then incubated with anti-fHbp (10 µg/ml), purified C1 complex (7.5 µg/ml) and pure C4 (50 µg/ml), and C4b deposited on the bacterial surface was measured by flow cytometry. Significantly less C4b was deposited on each of the Cap+ strains compared to its corresponding Cap− mutant (Fig. 2A; representative histogram tracings are shown in Supplemental Fig. S1). C4b deposition was dependent on the presence of specific Ab, as revealed by minimal C4b deposition when bacteria were incubated with pure C1 and C4 alone (Fig. 2B). Considerable variation in the amount of C4b deposited across the Cap− mutants was noted; the least amount of C4b was deposited on the group A Cap− mutant, intermediate amounts on the group B Cap-mutant, while high levels of C4b were deposited on the groups C, W and Y Cap− mutants. Bacteria were then incubated with anti-fHbp and IgG/IgM-depleted normal human serum and C4b deposition was measured. With the exception of the group A mutant pair where similar and low amounts of C4b was deposited, inhibition of C4b deposition by capsular polysaccharide mirrored findings with purified C1 and C4 (Figs. 2C). Similar to observations in Fig. 2B, minimal C4b was deposited when anti-fHbp was excluded from the reaction mixture (≤10% of the fluorescence seen when anti-fHbp was present in the reaction mixture; data not shown). We also performed western blotting analysis of bacteria that were incubated with anti-fHbp and purified C1 and C4 (Fig. 2D), which confirmed findings of the flow cytometry assays using a second independent method. It is worth noting that the targets for C4b deposited by anti-fHbp were LOS and opacity protein (Opa), as described previously in studies using normal human serum (41). Collectively, the data suggest that all five major meningococcal capsular polysaccharides interfere with C4b deposition mediated by an anti-fHbp mAb.
Capsule interferes with C1q engagement by anti-fHbp
C4b deposition on a surface is the result of several steps. C1 complex binding by Ab induces a conformational change in C1q, which results in autoactivation of C1r. Activated C1r then cleaves and activates C1s and the latter cleaves a 9 kD fragment from the amino terminus of the α-chain of C4, called C4a, which exposes an internal thioester bond in C4. Nucleophilic attack of the exposed and metastable thioester in the C4b fragment by hydroxyl or amino groups leads to formation of covalent ester or amide bonds, respectively (43, 44).
The ability of anti-fHbp to engage C1q represents an early step in Ab-mediated CP activation and was studied next. As shown in Fig. 3A (Supplemental Fig. S2 shows representative histograms), capsule expression interfered with C1q binding by anti-fHbp. The amount of C1q bound also varied greatly across Cap− mutants and showed a statistically significant positive correlation with the amount of anti-fHbp -mediated C4b deposition (Fig. 3B). Collectively, these data show that despite similar anti-fHbp binding, the activity of anti-fHbp to activate the CP and deposit C4b varies across strains.
Effects of capsular polysaccharide expression on CP-mediated killing of meningococci by anti-fHbp
We next determined the ability of anti-fHbp to mediate complement-dependent bactericidal activity through the classical pathway. Anti-factor Bb mAb (100 µg/ml) was added to IgG/IgM-depleted serum to block the alternative pathway of complement (40). As shown in Fig. 4, the groups B, C, W and Y encapsulated mutants were significantly more resistant to CP-mediated killing by the anti-fHbp mAb. Paradoxically, the Cap− group A mutant was more resistant than the isogenic Cap+ mutant. Controls where bacteria were incubated with complement (IgG/IgM depleted serum) alone resulted in 120 – 200% survival in every instance (data not shown).
Capsule inhibits C4b deposition by anti-PorA mAbs
To ensure that the effects of capsular polysaccharide on CP activation was not specific for anti-fHbp Ab, we next measured C4b deposition mediated by anti-PorA mAbs. mAbs that were available against four of the strains used in this study (groups A, B, C and Y) were tested for their ability to deposit C4b. While capsule expression did not affect binding of the mAbs to the bacterial surface (Fig. 5A), decreased C4b deposition was noted on the Cap+ mutants (Fig. 5B). Taken together, these data suggest that capsule limits C4b deposition by specific IgG mAbs.
Capsule inhibits CP activation by IgM directed against LOS
IgM is very efficient in fixing complement because a single IgM molecule can engage C1. However, IgM (Mr ~900 kD) is considerably larger than IgG (Mr ~150 kD) and the effects of capsule on binding and activity of IgM directed against noncapsular membrane antigens is not clear and was investigated next.
Anti-LOS IgM mAb directed against the unsialylated lacto-N-neotetraose structure that was elaborated by all strains used in this study was tested for its ability to bind to the Cap+ and Cap− mutants. At all dilutions tested, we noted a trend toward lower binding of mAb 3F11 to the Cap+ mutants (Fig. 6A). The differences were the least with the group A mutant pair (~1.2-fold), and the highest (~2-fold) with the group B strain pair.
To determine whether capsule interfered with C4b deposition by IgM mAb 3F11, we first ‘normalized’ the amount of 3F11 binding to Cap+ and Cap− mutants. Based on results above, a 2-fold higher concentration of 3F11 supernatant was used with each Cap+ mutant compared to the Cap− mutant and represented a conservative estimate to normalize binding. Two doses of mAb 3F11 were tested; the ‘high dose’ compared 1:8 and 1:16 dilutions, and the low dose compared 1:16 and 1:32 dilutions of 3F11 supernatant across the Cap+ and Cap− mutants, respectively. Again, in every instance, capsule expression was associated with lower C4b deposition (Fig. 6B).
Discussion
A novel finding in this study was that all five major meningococcal capsules interfered with engagement of pure C1q by IgG Fc, which led to decreased C4b deposition and inhibition of CP activation. We confirmed reduced C4b deposition using specific Abs directed against distinct antigens on five meningococcal groups. These observations may explain, at least in part, how capsular polysaccharides contribute to inhibition of the classical pathway. We emphasize that attenuation of the CP by capsule is stoichiometric and can be overcome as the titers of Ab and complement exceed a threshold, as occurs following immunization.
In a previous study we showed that the five meningococcal capsular groups interacted with the alternative pathway of complement in different ways (33). Groups B and C capsules inhibited the human alternative pathway, the group A capsule had no effect, while the groups W and Y capsules enhanced alternative pathway activation. Paradoxical enhancement of alternative pathway activation on the groups W and Y strains would theoretically place these strains at a disadvantage were complement activation to proceed in an unimpeded manner. However, the site of complement activation may be a critical factor in determining whether C5b-9 is generated and if the generated C5b-9 is bactericidal. Smooth LPS expressed by Klebsiella pneumoniae results in C3b deposited at locations too distant from the membrane to permit generated C5b-9 to elicit bactericidal activity (45). Elegant studies by Joiner and colleagues showed that similar amounts of C3 and C9 were deposited on Escherichia coli O111B4 incubated with complement alone compared to E. coli presensitized with immune Ab plus complement, but bacterial killing was observed only in the presence of Ab or F(ab')2 (46). These data suggest that the location of complement activation plays a critical role in bacterial killing. The AP alone does not kill wild-type N. meningitidis, including groups W and Y isolates where capsule activates the AP and serves as a site for C3 fragment deposition (33). We speculate that the distal locations of C3b on capsule may not result in C5b-9 formation that inserts into the bacterial membrane to effectively facilitate killing. Further, Rawal and Pangburn have shown that AP C5 convertase (C3bC3b,Bb) has a 6- to 9-fold lower catalytic rate than CP C5 convertase (C4bC3b,2a) (47), which may explain at least in part the ineffectiveness of the AP alone in mediating killing of Neisseriae. The ability of groups W and Y capsules to regulate the CP that is triggered by Ab bound to membrane antigens provides them a means to reduce CP-mediated complement activation that may otherwise have resulted in effective membrane attack complex insertion and bacterial death.
None of the capsule groups interfered with binding of specific IgG Ab, which is consistent with prior data, where similar amounts of anti-fHbp bound to strains that expressed different amounts of capsule (48). McNeil et al tested diverse wild-type encapsulated strains and we have confirmed and extended their observations using isogenic mutant strains that differed only in capsule expression. While capsule did not affect the binding of specific high-affinity IgG mAbs to their targets (in this instance, fHbp and PorA), it is not clear whether capsule would limit binding of lower affinity IgG Abs, for example, ‘natural’ Abs present in unimmunized individuals. By contrast, capsule expression decreased binding of an anti-LOS IgM mAb and the extent of inhibition varied across strains. Capsule likely poses an impediment to the bulky (~900 kD) pentameric IgM molecule from binding to its LOS epitope. A previous study showed that group B capsule expression reduced the binding of another large protein, C4b binding protein (C4BP; Mr ~470 kD) to bacteria by about 50% (49).
In a similar manner, capsule may also decrease engagement of the relatively large C1q molecule (~460 kD) by anti-fHbp Fc. C1q is formed by 18 polypeptide chains (6 A, B and C chains) that are organized into 6 subunits, each comprising an A, B and C chain. Engagement of C1q by IgG is a complex event. Recent data have shown that the IgG bound to surfaces form ordered Ab hexamers through noncovalent interactions of Fc, which results in binding of activated C1 (50). Further, the interaction between the globular head of C1q and IgG interaction is ionic in nature (51) and interactions between charged amino acids contribute to C1q-IgG binding (52–54). How capsules interfere with C1q engagement by Ab remains unclear. Based on published literature, possibilities include restriction of IgG hexamer formation and/or interference of ionic interactions between C1q and IgG by the negatively charged capsules.
Another interesting finding was that the major targets for C4b deposition when Ab was specifically directed against fHbp were LOS and Opa, similar to that we have described previously when normal human serum that contained ‘natural’ Ab was used to activate complement and deposit C4b (41). We have shown previously that groups W and Y capsules themselves serve as targets for C3 fragments (33). Akin to C3b, C4b (derived from the C4B isoform of C4) also forms covalent ester binds with electron-donating –OH groups (43, 44). Whether –OH groups of glycans in capsular polysaccharides also serve as acceptors for C4b when complement is activated in close proximity, for example by anti-capsular Ab, merits further study.
Uria et al showed that group C strains of N. meningitidis that expressed high levels of capsule because of the presence of an insertion sequence (IS1301) between the capsule biosynthesis and transport operons were resistant to killing by vaccine-elicited anti-capsular Ab (55). This finding appeared seemingly counterintuitive because of greater expression of the target antigen would have permitted higher levels of Ab binding and more killing. As stated above, group C capsule regulates the alternative pathway and up-regulation of capsule could dampen alternative pathway amplification initiated by the CP. Other possibilities such as activation of complement relatively distal to the membrane, which prevents insertion of ‘bactericidal’ membrane attack complex, may have also contributed to serum resistance. These capsule ‘hyperproducers’ were also resistant to killing by an anti-PorA mAb P1.5 (55); although the reason cited was inhibition of the alternative pathway, an additional but not mutually exclusive explanation is capsule-mediated CP inhibition as we have reported here. Whether complement activation by anti-capsular Ab and Ab directed against membrane antigens differ quantitatively or qualitatively is an important consideration from the perspective of vaccine development.
An additional important finding that emerged from this study was the wide variation in the amount of C4b deposited by anti-fHbp across the five unencapsulated meningococcal mutants, despite similar amounts of Ab binding. Levels of NspA expression (20) and different PorB alleles (19) are two variables that modulate alternative pathway activity, which necessitated the use of a complement source devoid of alternative pathway activity (achieved in this instance by blocking factor B function) to interpret results of serum bactericidal assays as they related to CP activation. With the exception of the group A mutants, capsule expression decreased CP-dependent killing by anti-fHbp. The reason for the greater (and unexpected) resistance to killing of the Cap− group A mutant relative to its Cap+ counterpart (Fig. 4) remains unclear, but it is worth noting that inhibition of C4b by group A capsule that was seen in the presence of anti-fHbp, pure C1 and pure C4 (Fig. 2A) was not seen in the presence of anti-fHbp and IgG/IgM depleted serum (Fig. 2C). Complement deposition and bactericidal activity in the context of serum requires participation of several proteins of the complement cascade and is modulated by complement inhibitors, which underscores the importance of corroborating data obtained using pure complement components with serum. Variation in the amount of C4b deposited and CP-mediated killing across strains despite similar amounts of anti-fHbp binding has important implications for the activity and evaluation of efficacy of fHbp-based vaccines. As an example, these data suggest that binding of anti-fHbp Ab alone may not predict the amount of CP activation and/or complement-dependent bacterial killing on different strains. The factor(s) that contribute to variations in C4b deposition among the strains remain to be elucidated. An example of surface variation that lead to differences in complement activation was described by Brown et al, where C2 cleavage varied markedly across human, guinea pig and sheep erythrocytes that were all sensitized with similar amounts of C1 and C4 (56).
Direct binding of C1 to several bacterial species can trigger CP activation independently of Ab (57–62). However, in this study only a low amount of C4b was deposited on meningococci that were incubated with C1 and C4 in the absence of Ab (Fig. 2B). These data are consistent with a requirement for Ab to kill meningococci (complement alone does not kill the bacteria), which forms the basis of evaluation of Ab responses to immunization by the serum bactericidal assay to predict protection against invasive disease (3).
In conclusion, we have shown that meningococcal capsular polysaccharides attenuate the CP of complement. These data highlight how this key virulence factor can modulate multiple facets of the complement cascade to subvert host immune responses.
Supplementary Material
Acknowledgements
We thank Dr. Ulrich Vogel, Universität Würzburg, Würzburg, Germany for providing the following strains: A2594 and its mynB mutant, H44/76, C2120, W171 and Y2225 and their lst and siaD lst mutants. We thank Dr. Dan M. Granoff (Childrens Hospital Oakland Research Institute) for providing mAb JAR 1 and Dr. Sunita Gulati (University of Massachusetts Medical School) for providing mAb 3F11 supernatants.
This work was supported by National Institutes of Health grants AI054544, AI084048 and AI32725.
Abbreviations used in this article
- LOS
lipooligosaccharide
- NHS
normal human serum
- HBSS
Hanks Balanced Salt Solution
- lst
lipooligosaccharide sialyltransferase
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