Figure 6. Suppression of MST1 expression causes androgen independence and resistance to MYC or EZH2 inhibitor-induced growth retardation. (A and B) Effects of MST1 knockdown on an androgen-independent LNCaP cell growth in vitro. LNCaP cells were transiently transfected with the pool of mock siRNA or MST1 siRNA. WB (A) and MTS assays (B) were performed at 48 h post-transfection in serum-starved growth conditions. (C) Analysis of cell growth by MTS assay in C4–2 cells treated with doses (0, 125, 250, 500, 750 or 1000 nM) of JQ1 in serum-fed conditions for 48h. (D and E) Analysis of C4–2 cell growth by MTS. C4–2 cells were transiently transfected with scramble (Src) shRNA and the MST1 shRNA, followed by treatment with doses (0, 40, or 60 µM) of 10058-F4 in serum-fed conditions for 48h (C) or with DMSO (vehicle) or 2 µM GSK126 for 72h (D). Data (± S.E.) are of multiple repeats. (F) A proposed model summarizing the findings.