Table 3. Primer sets and PCR conditions used in the present study.

	Forward primer	Reverse primer	Amplification reaction
Primer set 2*	Primer 5.1: (5″-AAG GAT AAC TCT TGT TAA TTG CAG-3″)	Primer 3.2: (5″-TGT CTA AAT TAC CCC AAT TTC C-3″)	30 cycles of 94 ° C, 57 ° C, and 72 ° C each for 30 s, followed by a final 2 min at 72 ° C
Primer set 3*	Primer 5.2: (5″-GGA ATA GCT TTT TGA GAA GAA GG-3″)	Primer 3.2: (5″-TGT CTA AAT TAC CCC AAT TTC C-3″)	30 cycles of 94 ° C, 57 ° C, and 72 ° C each for 30 s, followed by a final 2 min at 72 ° C
E. histolytica
	RRH5: (5″-GCG CCT TTT TAT TCA ATA TAC TCC-3″)	RRH3: (5″-GGA TGA AGA TAT CTT CAC AGG G-3″)	30 cycles of 94 ° C, 59 ° C , and 72 ° C each for 30 s, followed by a final 2 min at 72 ° C
E. dispar
	RRD5: (5″-CAT GAG GCG CCT TTT TAT CA-3″)	RRD3: (5″-AGG GGA TGA TGA TAT TGA ACA CAC TC-3″)	30 cycles of 94 ° C, 59 ° C , and 72 ° C each for 30 s, followed by a final 2 min at 72 ° C

Notes.

^*Two primer sets were used to target uninucleated cyst-producing Entamoeba species (E Victory, pers. comm., 2010).