Fig. 6.

Antibody depletion of CD8⁺ cells restores orthotopic tumor growth in myeloid-COX-2 KO mice. (A) Depletion of CD4⁺ (right) or CD8⁺ (left) T cells in mice treated with an anti (α)-CD4 or α-CD8 antibody was confirmed by flow cytometry of red blood cell-lysed whole blood (n = 3). (B) Depletion of CD4⁺ T cells did not significantly alter tumor growth in myeloid-COX-2 KO or WT mice (n = 6). (C) Depletion of CD8⁺ T cells increased tumor growth in WT mice and restored tumor growth in myeloid-COX-2 KO to WT levels (n = 6). Data are mean ± SEM. ^aComparison between myeloid-COX-2 KO isotype versus myeloid-COX-2 α-CD8; ^bComparison between WT isotype versus myeloid-COX-2 KO α-CD8, *P < 0.05, **P < 0.01, n.s. = not significant.