Table S1. Data for whole-lysosome conductance calculation.
| Experimental
conditions |
V m (mV) | I (pA) | g WL (pS) | |
|---|---|---|---|---|
| [Ca 2+] i | 60 mM | 100 | 15 | 265 |
| [Ca 2+] o | 2 mM | 60 | 5 | 301 |
| [K +] i | 70 mM | 20 | -7 | 300 |
| [K +] o | 60 mM | -20 | -20 | 316 |
| T | 296.16 K (23°C) | -60 | -35 | 339 |
| P Ca/ P K | 1000 | -100 | -50 | 349 |
| R | 8.3 J/(mol K) | |||
| F | 9.65 × 10 4 J/(V mol) |
Data on experimental conditions by Schieder et al. [ 1], used to calculate whole-lysosome TPC2-mediated Ca 2+ conductance, g WL, as explained in the text. The subscripts “i” and “o” refer to the inside and outside of a lysosome, respectively.