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. Author manuscript; available in PMC: 2014 Aug 11.
Published in final edited form as: Glia. 2013 Aug 5;61(10):1687–1697. doi: 10.1002/glia.22549

FIGURE 4.

FIGURE 4

After acute light lesion, Müller glia in tgif1−/− fish express a dedifferentiation marker similar to tgif1+/+ but misre-gulate Smad2/3 target genes. (A) BLBP and PCNA immunoreac-tivity in tgif1+/+ and tgif1−/− fish at 3 dpl in the lesioned area, as determined by the absence of cone nuclei. (B–E) qRT-PCR of whole retina extracts (retinas pooled from individual fish, n=3 replicates). We normalized gene expression to gpia and calculated fold change relative to the average tgif1+/+ 0 dpl level. * P<0.05, Student t-test comparing genotypes at the same lesion interval. Abbreviations as in Fig. 1.