Skip to main content
. Author manuscript; available in PMC: 2014 Aug 11.
Published in final edited form as: Nature. 2013 Nov 27;504(7478):143–147. doi: 10.1038/nature12830

Figure 2. NrasG12D/+ increased HSC and MPP self-renewal.

Figure 2

a) Secondary transplantation (n=19 recipients/genotype) of 3×106 bone marrow cells from primary recipient mice in Figure 1c (n=4 donors/genotype). Donor cell reconstitution in the myeloid (Mac-1+), B (B220+), and T (CD3+) cell lineages for 4 to 20 weeks after transplantation b) Transplantation of 3×106 bone marrow cells from secondary recipient mice in Figure 2a (n=3 donors/genotype) into tertiary recipient mice (n=8 recipients for control and 9 recipients for NrasG12D/+) c) Transplantation of 3×106 bone marrow cells from tertiary recipient mice (n=3 donors for control and 4 donors for NrasG12D/+) in Figure 2b into quaternary recipient mice (n=7 recipients for control and 17 for NrasG12D/+). Each serial transplant was performed at 20 weeks after the prior round of transplantation. Data represent mean±s.d.. Two-tailed student's t-tests were used to assess statistical significance. *P<0.05, **P<0.01, ***P<0.001.