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. 1995 Aug 15;92(17):8078–8082. doi: 10.1073/pnas.92.17.8078

Induction of antigen-specific cytolytic T cells in situ in human melanoma by immunization with synthetic peptide-pulsed autologous antigen presenting cells.

B Mukherji 1, N G Chakraborty 1, S Yamasaki 1, T Okino 1, H Yamase 1, J R Sporn 1, S K Kurtzman 1, M T Ergin 1, J Ozols 1, J Meehan 1, et al.
PMCID: PMC41290  PMID: 7644541

Abstract

Human melanoma cells can process the MAGE-1 gene product and present the processed nonapeptide EADPTGHSY on their major histocompatibility complex class I molecules, HLA-A1, as a determinant for cytolytic T lymphocytes (CTLs). Considering that autologous antigen presenting cells (APCs) pulsed with the synthetic nonapeptide might, therefore, be immunogenic, melanoma patients whose tumor cells express the MAGE-1 gene and who are HLA-A1+ were immunized with a vaccine made of cultured autologous APCs pulsed with the synthetic nonapeptide. Analyses of the nature of the in vivo host immune response to the vaccine revealed that the peptide-pulsed APCs are capable of inducing autologous melanoma-reactive and the nonapeptide-specific CTLs in situ at the immunization site and at distant metastatic disease sites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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