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. Author manuscript; available in PMC: 2015 Jul 21.
Published in final edited form as: Chembiochem. 2014 Jun 24;15(11):1563–1568. doi: 10.1002/cbic.201402059

Figure 1.

Figure 1

Yuh1 catalyzes the C-terminal exchange between D77 and amine-biotin I. (A) HPLC traces showing the formation of transamidation product (Ub-AB) and hydrolysis product (Ub) as a function of Yuh1 concentration. Reaction conditions: Ub-D77 (750 µM), amine-biotin I (250 mM), Yuh1 (indicated in plot), pH 10.4, RT, overnight. (B) The isotopic distribution of Ub-AB in a single charge state (z=9 or M9+). Red circles correspond to the theoretical distribution of isotopic abundance. Calc’d: calculated most abundance molecular weight. Expt’l: experimental most abundant molecular weight.