Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Oct;76(10):4793–4797. doi: 10.1073/pnas.76.10.4793

Sequences of vesicular stomatitis virus RNA in the region coding for leader RNA, N protein mRNA, and their junction.

D J Rowlands
PMCID: PMC413023  PMID: 228265

Abstract

The RNAs extracted from purified preparations of the Indiana and New Jersey serotypes of vesicular stomatitis virus were polyadenylylated in vitro by using polynucleotide phosphorylase and sequence determination was carried out by the dideoxynucleotide method using reverse transcriptase and dT8AC primer. On both virus RNAs a short stretch of adenylic acid residues is present between the regions coding for the leader and N protein mRNAs. Other features of the RNA sequences of the two viruses are compared to each other and to published data.

Full text

PDF
4793

Images in this article

4795Image
on p.4795
4795Image
on p.4795

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham G., Banerjee A. K. Sequential transcription of the genes of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1976 May;73(5):1504–1508. doi: 10.1073/pnas.73.5.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ball L. A., White C. N. Order of transcription of genes of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1976 Feb;73(2):442–446. doi: 10.1073/pnas.73.2.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Colonno R. J., Abraham G., Banerjee A. K. Blocked and unblocked 5'termini in vesicular stomatitis virus product RNA in vitro: their possible role in mRNA biosynthesis. Prog Nucleic Acid Res Mol Biol. 1976;19:83–87. doi: 10.1016/s0079-6603(08)60909-5. [DOI] [PubMed] [Google Scholar]
  4. Colonno R. J., Banerjee A. K. Complete nucleotide sequence of the leader RNA synthesized in vitro by vesicular stomatitis virus. Cell. 1978 Sep;15(1):93–101. doi: 10.1016/0092-8674(78)90085-5. [DOI] [PubMed] [Google Scholar]
  5. Colonno R. J., Banerjee A. K. Nucleotide sequence of the leader RNA of the New Jersey serotype of vesicular stomatitis virus. Nucleic Acids Res. 1978 Nov;5(11):4165–4176. doi: 10.1093/nar/5.11.4165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doyle M., Holland J. J. Prophylaxis and immunization in mice by use of virus-free defective T particles to protect against intracerebral infection by vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2105–2108. doi: 10.1073/pnas.70.7.2105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Engel J. D., Davidson N. Addition of poly(adenylic acid) to RNA using polynucleotide phosphorylase: an improved method for electron microscopic visualization of RNA-DNA hybrids. Biochemistry. 1978 Sep 5;17(18):3883–3888. doi: 10.1021/bi00611a032. [DOI] [PubMed] [Google Scholar]
  8. Hamlyn P. H., Browniee G. G., Cheng C. C., Gait M. J., Milstein C. Complete sequence of constant and 3' noncoding regions of an immunoglobulin mRNA using the dideoxynucleotide method of RNA sequencing. Cell. 1978 Nov;15(3):1067–1075. doi: 10.1016/0092-8674(78)90290-8. [DOI] [PubMed] [Google Scholar]
  9. Keene J. D., Schubert M., Lazzarini R. A., Rosenberg M. Nucleotide sequence homology at the 3' termini of RNA from vesicular stomatitis virus and its defective interfering particles. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3225–3229. doi: 10.1073/pnas.75.7.3225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Perrault J. Cross-linked double-stranded RNA from a defective vesicular stomatitis virus particle. Virology. 1976 Apr;70(2):360–371. doi: 10.1016/0042-6822(76)90278-6. [DOI] [PubMed] [Google Scholar]
  12. Porter A. G., Fellner P., Black D. N., Rowlands D. J., Harris T. J., Brown F. 3'-Terminal nucleotide sequences in the genome RNA of picornaviruses. Nature. 1978 Nov 16;276(5685):298–301. doi: 10.1038/276298a0. [DOI] [PubMed] [Google Scholar]
  13. Richardson C. C. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158–165. doi: 10.1073/pnas.54.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rose J. K. Nucleotide sequences of ribosome recgonition sites in messenger RNAs of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3672–3676. doi: 10.1073/pnas.74.9.3672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Villarreal L. P., Breindl M., Holland J. J. Determination of molar ratios of vesicular stomatitis virus induced RNA species in BHK21 cells. Biochemistry. 1976 Apr 20;15(8):1663–1667. doi: 10.1021/bi00653a012. [DOI] [PubMed] [Google Scholar]
  17. Zimmern D., Kaesberg P. 3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4257–4261. doi: 10.1073/pnas.75.9.4257. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES