Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Oct;76(10):4813–4816. doi: 10.1073/pnas.76.10.4813

Concanavalin A stimulates phospholipid methylation and phosphatidylserine decarboxylation in rat mast cells.

F Hirata, J Axelrod, F T Crews
PMCID: PMC413027  PMID: 92021

Abstract

When histamine release from rat peritoneal mast cells is stimulated by concanavalin A, membrane phospholipids are methylated in the early stage of this process. Exogenously added phosphatidylserine enhances the concanavalin A-induced histamine release, and at the same time the lectin markedly stimulates the decarboxylation and methylation of phosphatidylserine. Within minutes after the addition of concanavalin A to rat mast cells, the newly methylated phospholipids begin to disappear and an increased formation of lysophosphatidylcholine is observed. When rat mast cells are treated with concanavalin A in the absence of Ca2+, phospholipid methylation is stimulated but no significant release of histamine is detected. The subsequent exposure of the pretreated cells to Ca2+ causes increased release of histamine and degradation of methylated phospholipids. The inhibition of either synthesis or degradation of methylated phospholipids results in the inhibition of histamine release. These observations suggest that the synthesis and degradation of methylated lipids are an intrinsic part of the biochemical mechanism that modulate histamine release from mast cells.

Full text

PDF
4813

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chi E. Y., Lagunoff D., Koehler J. K. Freeze-fracture study of mast cell secretion. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2823–2827. doi: 10.1073/pnas.73.8.2823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chiang P. K., Cantoni G. L., Bader J. P., Shannon W. M., Thomas H. J., Montgomery J. A. Adenosylhomocysteine hydrolase inhibitors: synthesis of 5'-deoxy-5'-(isobutylthio)-3-deazaadenosine and its effect on Rous sarcoma virus and Gross murine leukemia virus. Biochem Biophys Res Commun. 1978 May 30;82(2):417–423. doi: 10.1016/0006-291x(78)90892-6. [DOI] [PubMed] [Google Scholar]
  3. GIACOBINI E., SEDVALL G., UVNAES B. PHOSPHATIDASE A ACTIVITY OF ISOLATED RAT MAST CELLS AND OF RED BLOOD CELLS, WHITE BLOOD CELLS AND SPINAL GANGLION CELLS. Exp Cell Res. 1965 Feb;37:368–375. doi: 10.1016/0014-4827(65)90185-0. [DOI] [PubMed] [Google Scholar]
  4. GOTH A., KNOOHUIZEN M. Tissue thromboplastins and histamine release from mast cells. Life Sci. 1962 Sep;1:459–465. doi: 10.1016/0024-3205(62)90053-x. [DOI] [PubMed] [Google Scholar]
  5. Goth A., Adams H. R., Knoohuizen M. Phosphatidylserine: selective enhancer of histamine release. Science. 1971 Sep 10;173(4001):1034–1035. doi: 10.1126/science.173.4001.1034. [DOI] [PubMed] [Google Scholar]
  6. Goth A., Johnson A. R. Current concepts on the secretory function of mast cells. Life Sci. 1975 Apr 15;16(8):1201–1213. doi: 10.1016/0024-3205(75)90305-7. [DOI] [PubMed] [Google Scholar]
  7. Hirata F., Axelrod J. Enzymatic methylation of phosphatidylethanolamine increases erythrocyte membrane fluidity. Nature. 1978 Sep 21;275(5677):219–220. doi: 10.1038/275219a0. [DOI] [PubMed] [Google Scholar]
  8. Hirata F., Axelrod J. Enzymatic synthesis and rapid translocation of phosphatidylcholine by two methyltransferases in erythrocyte membranes. Proc Natl Acad Sci U S A. 1978 May;75(5):2348–2352. doi: 10.1073/pnas.75.5.2348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hirata F., Corcoran B. A., Venkatasubramanian K., Schiffmann E., Axelrod J. Chemoattractants stimulate degradation of methylated phospholipids and release of arachidonic acid in rabbit leukocytes. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2640–2643. doi: 10.1073/pnas.76.6.2640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirata F., Strittmatter W. J., Axelrod J. beta-Adrenergic receptor agonists increase phospholipid methylation, membrane fluidity, and beta-adrenergic receptor-adenylate cyclase coupling. Proc Natl Acad Sci U S A. 1979 Jan;76(1):368–372. doi: 10.1073/pnas.76.1.368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ishizaka T., Ishizaka K. Triggering of histamine release from rat mast cells by divalent antibodies against IgE-receptors. J Immunol. 1978 Mar;120(3):800–805. [PubMed] [Google Scholar]
  12. KREMZNER L. T., WILSON I. B. A procedure for the determination of histamine. Biochim Biophys Acta. 1961 Jun 24;50:365–367. doi: 10.1016/0006-3002(61)90339-0. [DOI] [PubMed] [Google Scholar]
  13. Keller R. Concanavalin A, a model "antigen" for the in vitro detection of cell-bound reaginic antibody in the rat. Clin Exp Immunol. 1973 Jan;13(1):139–147. [PMC free article] [PubMed] [Google Scholar]
  14. Martin T. W., Lagunoff D. Inhibition of mast cell histamine secretion by N-substituteed derivatives of phosphatidylserine. Science. 1979 May 11;204(4393):631–633. doi: 10.1126/science.86210. [DOI] [PubMed] [Google Scholar]
  15. Martin T. W., Lagunoff D. Interaction of phosphatidylserine with mast cells. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4997–5000. doi: 10.1073/pnas.75.10.4997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Orr T. S., Cox J. S. Disodium cromoglycate, an inhibitor of mas cell degranulation and histamine release induced by phospholipase A. Nature. 1969 Jul 12;223(5202):197–198. doi: 10.1038/223197b0. [DOI] [PubMed] [Google Scholar]
  17. Segal D. M., Taurog J. D., Metzger H. Dimeric immunoglobulin E serves as a unit signal for mast cell degranulation. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2993–2997. doi: 10.1073/pnas.74.7.2993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Whelan C. J. Histamine release from rat peritoneal mast cells by phospholipase A. The "activation" of phospholipase A by phospholipids. Biochem Pharmacol. 1978;27(17):2115–2118. doi: 10.1016/0006-2952(78)90282-4. [DOI] [PubMed] [Google Scholar]
  19. Yorio T., Bentley P. J. Phospholipase A and the mechanism of action of aldosterone. Nature. 1978 Jan 5;271(5640):79–81. doi: 10.1038/271079a0. [DOI] [PubMed] [Google Scholar]
  20. Zimmerman T. P., Wolberg G., Duncan G. S. Inhibition of lymphocyte-mediated cytolysis by 3-deazaadenosine: evidence for a methylation reaction essential to cytolysis. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6220–6224. doi: 10.1073/pnas.75.12.6220. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES